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Team:UC Chile/Cyanolux/Biobricks
From 2012.igem.org
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<h2>BBa_K743009</h2> | <h2>BBa_K743009</h2> | ||
| - | pSB1C3_IntK(BBa_K743006) with mRFP1 under PpsaAB promoter. | + | pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsaAB promoter. |
[[File:UC Chile-BBa_K743009.jpg | 500px ]] | [[File:UC Chile-BBa_K743009.jpg | 500px ]] | ||
<h2>BBa_K743016</h2> | <h2>BBa_K743016</h2> | ||
| - | pSB1C3_IntK(BBa_K743006) with mRFP1 under PpsbA2 promoter. | + | pSB1C3_IntK(BBa_K743006) with mRFP1 under <i>Synechocystis</i> PpsbA2 promoter. |
[[File:UC Chile-BBa_K743016.jpg | 500px ]] | [[File:UC Chile-BBa_K743016.jpg | 500px ]] | ||
<h2>BBa_K743014</h2> | <h2>BBa_K743014</h2> | ||
| - | pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus | + | pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus luminescent under <i>Synechocystis</i> Transaldolase promoter. |
[[File:UC Chile-IntKplasmidLuxABxl.jpg | 600px ]] | [[File:UC Chile-IntKplasmidLuxABxl.jpg | 600px ]] | ||
<h2>BBa_K743015</h2> | <h2>BBa_K743015</h2> | ||
| - | pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under Transaldolase promoter. | + | pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under <i>Synechocystis</i> Transaldolase promoter. |
[[File:UC Chile-IntKfullplasmidResult.jpg | 600px ]] | [[File:UC Chile-IntKfullplasmidResult.jpg | 600px ]] | ||
<h2>BBa_K743018</h2> | <h2>BBa_K743018</h2> | ||
| - | pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under Transaldolase promoter. | + | pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under <i>Synechocystis</i> Transaldolase promoter. |
[[File:UC Chile-BBa_K743018sfGFPtag.jpg | 600px ]] | [[File:UC Chile-BBa_K743018sfGFPtag.jpg | 600px ]] | ||
Revision as of 09:53, 26 October 2012
Contents |
Plasmid backbones
pSB1C3_IntK (BBa_K743006)
Neutral recombination plasmid backbone. Kanamycin resistance as a selectable marker for integration. EcoRI restriction site in RS2. Plasmid backbone designed for Gibson Assembly.
pSB1C3_IntS (BBa_K743010)
Suceptibility generating plasmid backbone. Spectinomycin resistance as a selectable marker for integration. mRFP1 under constitutive promoter in E.coli for negative selection of standard assembly.
Biobricks and Constructs
BBa_K743000
First recombination site of IntK plasmid backbone.
BBa_K743001
Second recombination site of IntK plasmid backbone. Illegal EcoRI restriction site present. As it always is used as a downstream part, illegal site does not interfere with Standard Assembly.
BBa_K743002
Synechocystis PCC. 6803 Citocrome C promoter.
BBa_K743009
pSB1C3_IntK(BBa_K743006) with mRFP1 under Synechocystis PpsaAB promoter.
BBa_K743016
pSB1C3_IntK(BBa_K743006) with mRFP1 under Synechocystis PpsbA2 promoter.
BBa_K743014
pSB1C3_IntK(BBa_K743006) with LuxAB genes from Photorhabdus luminescent under Synechocystis Transaldolase promoter.
BBa_K743015
pSB1C3_IntK(BBa_K743006) with LuxAB genes from Vibrio fisherii under Synechocystis Transaldolase promoter.
BBa_K743018
pSB1C3_IntK(BBa_K743006) with sfGFP gene with rapid degradation tag under Synechocystis Transaldolase promoter.
