Team:UCSF/Auxotroph Data

From 2012.igem.org

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<a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/auxotrophs/Kerner%202012%20PLoS%20ONE.pdf"><b>A Programmable <i>Escherichia coli</i> Consortium via Tunable Symbiosis </b> </a>. They showed some experimental analysis which were the first steps at showing that these strains could be induced at varying levels to get a range of ratios between the two strains. <br>
<a href="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/auxotrophs/Kerner%202012%20PLoS%20ONE.pdf"><b>A Programmable <i>Escherichia coli</i> Consortium via Tunable Symbiosis </b> </a>. They showed some experimental analysis which were the first steps at showing that these strains could be induced at varying levels to get a range of ratios between the two strains. <br>
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<img align="left" style="margin-bottom:8px; width:755px;height:410px; padding:0;" src="https:">
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<regulartext> We were able to create our own version of the model presented in the Kerner 2012 paper and obtain a model based on data that we collected.
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<regulartext> A 3D surface of that graph was obtained and a slice of the graph is shown here:
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<img align="left" style="margin-bottom:8px; width:555px;height:410px; padding:0;" src="https://dl.dropbox.com/u/24404809/iGEM%202012/igem%202012%20website%20photos/auxotrophs/slice.png">

Revision as of 02:29, 4 October 2012

Growth and Analysis of Auxotroph System in E. coli


The W3 and Y3 strains (tryptophan ad tyrosine auxotrophs) that we obtained from the Lin Lab at the University of Michigan had already been studied in a paper published earlier this year. A Programmable Escherichia coli Consortium via Tunable Symbiosis . They showed some experimental analysis which were the first steps at showing that these strains could be induced at varying levels to get a range of ratios between the two strains.
We were able to create our own version of the model presented in the Kerner 2012 paper and obtain a model based on data that we collected.

A 3D surface of that graph was obtained and a slice of the graph is shown here: