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Would any of your project ideas raise safety issues in terms of: researcher safety, public safety, or environmental safety?

Our project does not pose any dangers to researchers or the public: We are following widely used lab protocols in a appropriate lab facility. The organisms used in our project, namely E. coli (TOP10 strain) and S. cerevisiae, are well-known and considered safe.

Although, by building a biosensor we are tackling an environmental problem, our system is not intended to be used in the field but in a lab only.

Do any of the new BioBrick parts (or devices) that you made this year raise any safety issues?

We have designed 3 different types of BioBricks. Membrane bound receptors for sensing hormones, a small inverter system to regulate our reporter gene. None of these are found toxic or dangerous by any means.

Which specific biosafety rules or guidelines do you have to consider in your country?

In germany any work with genetically modified organisms is regulated by the "Gentechnikgesetz". There are different biosafety levels reaching from 1 to 4. Our lab has been registered as level 1. Our work matches the definition of level 1 since on our current understanding we see no threats on human health or the environment. Genetically modified organisms are allowed to be released into nature only on permission of the Umweltministerium and once freed need to be constantly monitored.

Do you have any other ideas how to deal with safety issues that could be useful for future iGEM competitions? How could parts, devices and systems be made even safer through biosafety engineering?