http://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&feed=atom&action=historyTeam:Tuebingen/Project - Revision history2024-03-29T00:50:29ZRevision history for this page on the wikiMediaWiki 1.16.0http://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=123974&oldid=prevJakobmatthes at 14:46, 18 September 20122012-09-18T14:46:34Z<p></p>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">== The Project ==</ins></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [[Team:Tuebingen/ProjectOverview|Overview]] <br /> A short introduction including an animation film.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* [[Team:Tuebingen/ProjectOverview|Overview]] <br /> A short introduction including an animation film.</div></td></tr>
</table>Jakobmattheshttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=123971&oldid=prevJakobmatthes at 14:46, 18 September 20122012-09-18T14:46:13Z<p></p>
<a href="http://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=123971&oldid=96030">Show changes</a>Jakobmattheshttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=96030&oldid=prevJakobmatthes: /* Mechanism */2012-09-05T10:20:14Z<p><span class="autocomment">Mechanism</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Mechanism''' ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== '''Mechanism''' ==</div></td></tr>
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<tr><td colspan="2"> </td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins style="color: red; font-weight: bold; text-decoration: none;">[[File:MechanismScheme.png|right|400px|Mechanism]]</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Igem_2.3.jpg|thumb|right|Planned mechanism]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Igem_2.3.jpg|thumb|right|Planned mechanism]]</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del style="color: red; font-weight: bold; text-decoration: none;">[[File:MechanismScheme.png|Mechanism]]</del></div></td><td colspan="2"> </td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Naturally occuring iron receptors of the PAQR family are found to repress</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Naturally occuring iron receptors of the PAQR family are found to repress</div></td></tr>
</table>Jakobmattheshttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=96029&oldid=prevJakobmatthes: /* Mechanism */2012-09-05T10:18:46Z<p><span class="autocomment">Mechanism</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Naturally occuring iron receptors of the PAQR family are found to repress</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Naturally occuring iron receptors of the PAQR family are found to repress</div></td></tr>
</table>Jakobmattheshttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=96024&oldid=prevJakobmatthes at 10:18, 5 September 20122012-09-05T10:18:00Z<p></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* It should be as cost-efficient as possible for easy and regular application</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* It should be as cost-efficient as possible for easy and regular application</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* It should be resistant to yeast's own metabolism (<del class="diffchange diffchange-inline">Not be disturbed </del>by unexpected <del class="diffchange diffchange-inline">occuring </del>expression).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* It should be resistant to yeast's own metabolism (<ins class="diffchange diffchange-inline">no disturbance </ins>by unexpected expression).</div></td></tr>
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<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>At first we had to find an appropriate receptor to <del class="diffchange diffchange-inline">"grab" </del>steroid</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>At first we had to find an appropriate receptor to <ins class="diffchange diffchange-inline">bind </ins>steroid</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>hormones in efluents. <del class="diffchange diffchange-inline"> This </del>should fulfill the following requirements:</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>hormones in efluents. <ins class="diffchange diffchange-inline">It </ins>should fulfill the following requirements:</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* It should only be responsible to substrates we wish to detect, so the results of the test will not be falsified.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* It should only be responsible to substrates we wish to detect, so the results of the test will not be falsified.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Igem_2.3.jpg|thumb|right|Planned mechanism]]</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>[[File:Igem_2.3.jpg|thumb|right|Planned mechanism]]</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Naturally occuring iron receptors of the PAQR family are found to repress</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Naturally occuring iron receptors of the PAQR family are found to repress</div></td></tr>
</table>Jakobmattheshttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=95965&oldid=prevJanjanjan: /* Mechanism */2012-09-05T09:49:35Z<p><span class="autocomment">Mechanism</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Naturally occuring iron receptors of the PAQR family are found to <del class="diffchange diffchange-inline">block</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Naturally occuring iron receptors of the PAQR family are found to <ins class="diffchange diffchange-inline">repress</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">FET3 </del>promotor on high <del class="diffchange diffchange-inline">level </del>of extra cellular iron. According to</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div><ins class="diffchange diffchange-inline">fet3 </ins>promotor on high <ins class="diffchange diffchange-inline">levesl </ins>of extra<ins class="diffchange diffchange-inline">-</ins>cellular iron. According to</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>[http://www.ncbi.nlm.nih.gov/pubmed/18603275 Smith <del class="diffchange diffchange-inline">JL </del>et al] <del class="diffchange diffchange-inline">expressed</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>[http://www.ncbi.nlm.nih.gov/pubmed/18603275 <ins class="diffchange diffchange-inline">J </ins>Smith et al<ins class="diffchange diffchange-inline">.</ins>]</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>human mPR in yeast induced the same signal <del class="diffchange diffchange-inline">ligating </del>to estrogen. Relying</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>human mPR <ins class="diffchange diffchange-inline">expressed </ins>in yeast induced the same signal <ins class="diffchange diffchange-inline">binding </ins>to estrogen. Relying</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>on this results we will try to express various mPRs of ''Danio rerio'',</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>on this results we will try to express various mPRs of ''Danio rerio'',</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>''Xanophus laevis'' in yeast which we find <del class="diffchange diffchange-inline">better </del>fitting to measure</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>''Xanophus laevis'' in yeast which we find fitting to measure</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>endocrine substances that influence fish.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>endocrine substances that influence fish.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We will transform the negative signal <del class="diffchange diffchange-inline">of FET3 </del>by <del class="diffchange diffchange-inline">letting it control an</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We will transform the negative signal <ins class="diffchange diffchange-inline">(fet3 repression) into a positive signal </ins>by <ins class="diffchange diffchange-inline">regulating a repressor </ins>(<ins class="diffchange diffchange-inline">rox1 or mig1</ins>) <ins class="diffchange diffchange-inline">with Pfet3</ins>. This <ins class="diffchange diffchange-inline">repressor </ins>will <ins class="diffchange diffchange-inline">in turn </ins>regulate the <ins class="diffchange diffchange-inline">expression </ins>of the reporter gene <ins class="diffchange diffchange-inline">(firefly luciferase or beta-galactosidase) </ins>and <ins class="diffchange diffchange-inline">allow quantitative measurement</ins>.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">inhibitor </del>(<del class="diffchange diffchange-inline">ROX1 and MIG1</del>). This <del class="diffchange diffchange-inline">inhibitor </del>will regulate the <del class="diffchange diffchange-inline">promotor </del>of</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">our reporter gene. For </del>the <del class="diffchange diffchange-inline">promoter of our </del>reporter gene <del class="diffchange diffchange-inline">we chose ANB1</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>and <del class="diffchange diffchange-inline">SUC2, because these are targets of ROX1 and MIG1. By negating the</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">negative signal of FET3 we hope to obtain a positive signal sensitive</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">enough to measure low concentrations of different endocrine substances</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Receptors ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Receptors ===</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>Membrane bound, <del class="diffchange diffchange-inline">7</del>-<del class="diffchange diffchange-inline">Transmembranereceptor (C-terminus inside,N-terminus</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>Membrane <ins class="diffchange diffchange-inline">progesterone receptors are membrane </ins>bound, <ins class="diffchange diffchange-inline">seven</ins>-<ins class="diffchange diffchange-inline">transmembrane receptors and belong to the </ins>PAQR family (progesterone adiponectin Q receptor)<ins class="diffchange diffchange-inline">. These </ins>G-<ins class="diffchange diffchange-inline">protein </ins>coupled <ins class="diffchange diffchange-inline">receptors activate inhibitory </ins>Gi units.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">outside), </del>PAQR family (progesterone adiponectin Q receptor)<del class="diffchange diffchange-inline">, Hly-III</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">superfamily </del>G-<del class="diffchange diffchange-inline">Protein </del>coupled<del class="diffchange diffchange-inline">: activation of inhibitori </del>Gi units<del class="diffchange diffchange-inline">:</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline">reduced adenylyl-cyclase activity</del>. <del class="diffchange diffchange-inline"> </del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Inhibitors and their targets ===</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>=== Inhibitors and their targets ===</div></td></tr>
</table>Janjanjanhttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=94757&oldid=prevRoMann: /* Occurring Questions */2012-09-04T13:06:46Z<p><span class="autocomment">Occurring Questions</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>our system:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>our system:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* It should be as cost-efficient as possible for easy and regular</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* It should be as cost-efficient as possible for easy and regular application</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>application</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* It should be resistant to yeast's own metabolism (Not be disturbed by unexpected occuring expression).</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* It should be resistant to yeast's own metabolism (Not be disturbed by</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>unexpected occuring expression).</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>At first we had to find an appropriate receptor to "grab" steroid</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>At first we had to find an appropriate receptor to "grab" steroid</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>hormones in efluents. This should fulfill the following requirements:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>hormones in efluents. This should fulfill the following requirements:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* It should only be responsible to substrates we wish to detect, so the</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* It should only be responsible to substrates we wish to detect, so the results of the test will not be falsified.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>results of the test will not be falsified.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* It has to be easiliy integrated into yeast's cell membrane.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>* It has to be easiliy integrated into yeast's cell membrane.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Its nucleic acid sequence should not be too long so we can put it onto</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* Its nucleic acid sequence should not be too long so we can put it onto a plasmid vector.</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div><del class="diffchange diffchange-inline"> </del>a plasmid vector.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We chose the membrane progesterone receptor of the zebra fish (''Danio</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We chose the membrane progesterone receptor of the zebra fish (''Danio rerio'') and the African clawed frog (''Xenopus laevis''). We focused on</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>rerio'') and the African clawed frog (''Xenopus laevis''). We focused on</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>these receptors, since they are easy to duplicate and interact with a</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>these receptors, since they are easy to duplicate and interact with a</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>broad bandwith of sex-determining hormones.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>broad bandwith of sex-determining hormones.</div></td></tr>
</table>RoMannhttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=94747&oldid=prevRoMann: /* Occurring Questions */2012-09-04T13:00:45Z<p><span class="autocomment">Occurring Questions</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>organisms:</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>organisms:</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Escherichia coli</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">''</ins>Escherichia coli<ins class="diffchange diffchange-inline">''</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>* Saccharomyces cerevisiae</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>* <ins class="diffchange diffchange-inline">''</ins>Saccharomyces cerevisiae<ins class="diffchange diffchange-inline">''</ins></div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Finally we decided for yeast, since it has been done more research with</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>Finally we decided for yeast, since it has been done more research with</div></td></tr>
</table>RoMannhttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=94740&oldid=prevRoMann: /* Occurring Questions */2012-09-04T12:59:29Z<p><span class="autocomment">Occurring Questions</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> a plasmid vector.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div> a plasmid vector.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>We chose the membrane progesterone receptor of zebra fish (''Danio</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>We chose the membrane progesterone receptor of <ins class="diffchange diffchange-inline">the </ins>zebra fish (''Danio</div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>rerio'') and <del class="diffchange diffchange-inline">of </del>African clawed frog (''<del class="diffchange diffchange-inline">Xenophus </del>laevis''). We focused on</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>rerio'') and <ins class="diffchange diffchange-inline">the </ins>African clawed frog (''<ins class="diffchange diffchange-inline">Xenopus </ins>laevis''). We focused on</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>these receptors, since they are easy to duplicate and interact with a</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>these receptors, since they are easy to duplicate and interact with a</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>broad bandwith of sex-determining hormones.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>broad bandwith of sex-determining hormones.</div></td></tr>
</table>RoMannhttp://2012.igem.org/wiki/index.php?title=Team:Tuebingen/Project&diff=94642&oldid=prevJanjanjan: /* Reporter genes */2012-09-04T11:29:12Z<p><span class="autocomment">Reporter genes</span></p>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>which amplifies our initial signal to allow a quantitative measurement.</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>which amplifies our initial signal to allow a quantitative measurement.</div></td></tr>
<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>The enzyme '''luciferase''' fulfills these conditions and is <del class="diffchange diffchange-inline">possible</del></div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>The enzyme '''luciferase''' fulfills these conditions and is <ins class="diffchange diffchange-inline">our</ins></div></td></tr>
<tr><td class='diff-marker'>-</td><td style="background: #ffa; color:black; font-size: smaller;"><div>candidate <del class="diffchange diffchange-inline">for our mechanism</del>.</div></td><td class='diff-marker'>+</td><td style="background: #cfc; color:black; font-size: smaller;"><div>candidate <ins class="diffchange diffchange-inline">of choice</ins>.</div></td></tr>
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<tr><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Implementation ==</div></td><td class='diff-marker'> </td><td style="background: #eee; color:black; font-size: smaller;"><div>== Implementation ==</div></td></tr>
</table>Janjanjan