Team:Tuebingen/NotebookAppendix
From 2012.igem.org
Appendix
To complete this report we list all products and software used over the course of our project.
Chemicals
We needed the following chemicals:
- Ampicillin
beta-lactam antibiotic
- Agarose
Polysaccharide, major component of Agar
- Dimethylsulfoxid (DMSO)
organic solvent
- Acetic Acid
- Ethylenediaminetetraacetic acid (EDTA)
- Ethanol
- TRIS
buffer solution for enzymes
- Nucleoside triphosphate
- Trypton
- Isopropanol
- Isopropyl-β-D-thiogalactopyranosid (IPTG)
- LB-medium
used for growth of E.coli
- Agar-Agar
Software
The following list of software was used in the team:
- Unipro UGENE
UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
- Vector NTI (commercial)
All our primers were designed using Vector NTI which is also used by our advisors.
- BLAST
BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
- Google Drive
Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).