Team:Tuebingen/NotebookAppendix

From 2012.igem.org

(Difference between revisions)
(Software)
 
(18 intermediate revisions not shown)
Line 1: Line 1:
{{:Team:Tuebingen/Template/Tuebingen}}
{{:Team:Tuebingen/Template/Tuebingen}}
 +
= Appendix =
 +
''To complete this report we list all products and software used over the course of our project.''
 +
== Constructs ==
 +
<table class="wikitable">
 +
<tr><th>backbone pRS#</th><th>part #1</th><th>part #2</th><th>part #3</th></tr>
 +
<tr><td>313</td><td>Padh1</td><td>mPR ''Danio rerio''</td><td>Tadh1</td></tr>
 +
<tr><td>313</td><td>Padh1</td><td>mPR ''Xenopus laevis''</td><td>Tadh1</td></tr>
 +
<tr><td>315</td><td>Pfet3</td><td>rox1</td><td>Tadh1</td></tr>
 +
<tr><td>315</td><td>Pfet3</td><td>mig1</td><td>Tadh1</td></tr>
 +
<tr><td>316</td><td>Panb1</td><td>lacZ</td><td>Tadh1</td></tr>
 +
<tr><td>316</td><td>Panb1</td><td>luciferase</td><td>Tadh1</td></tr>
 +
<tr><td>316</td><td>Psuc2</td><td>lacZ</td><td>Tadh1</td></tr>
 +
<tr><td>316</td><td>Psuc2</td><td>luciferase</td><td>Tadh1</td></tr>
 +
</table>
-
 
+
== Chemicals ==
-
=== Appendix ===
+
-
 
+
-
''To complete this report we list all products and software used over the course of our project.''
+
-
 
+
-
==== Chemicals ====
+
We needed the following chemicals:
We needed the following chemicals:
* Ampicillin <br /> beta-lactam antibiotic
* Ampicillin <br /> beta-lactam antibiotic
-
 
* Agarose <br /> Polysaccharide, major component of Agar
* Agarose <br /> Polysaccharide, major component of Agar
-
 
* Dimethylsulfoxid (DMSO) <br /> organic solvent
* Dimethylsulfoxid (DMSO) <br /> organic solvent
-
 
* Acetic Acid
* Acetic Acid
-
 
* Ethylenediaminetetraacetic acid (EDTA)
* Ethylenediaminetetraacetic acid (EDTA)
-
 
* Ethanol
* Ethanol
-
 
* TRIS <br /> buffer solution for enzymes
* TRIS <br /> buffer solution for enzymes
-
 
* Nucleoside triphosphate
* Nucleoside triphosphate
-
 
* Trypton
* Trypton
-
 
* Isopropanol
* Isopropanol
-
 
* Isopropyl-β-D-thiogalactopyranosid (IPTG)
* Isopropyl-β-D-thiogalactopyranosid (IPTG)
-
 
* LB-medium <br /> used for growth of E.coli
* LB-medium <br /> used for growth of E.coli
-
 
* Agar-Agar
* Agar-Agar
-
 
+
== Software ==
-
 
+
-
==== Software ====
+
The following list of software was used in the team:
The following list of software was used in the team:
Line 50: Line 45:
* [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] <br/> BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
* [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] <br/> BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
-
* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE]
+
* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE] <br/> The free and useful plasmid editor.
* [http://drive.google.com Google Drive] <br/> Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).
* [http://drive.google.com Google Drive] <br/> Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).

Latest revision as of 12:53, 26 September 2012



Contents

Appendix

To complete this report we list all products and software used over the course of our project.

Constructs

backbone pRS#part #1part #2part #3
313Padh1mPR Danio rerioTadh1
313Padh1mPR Xenopus laevisTadh1
315Pfet3rox1Tadh1
315Pfet3mig1Tadh1
316Panb1lacZTadh1
316Panb1luciferaseTadh1
316Psuc2lacZTadh1
316Psuc2luciferaseTadh1

Chemicals

We needed the following chemicals:

  • Ampicillin
    beta-lactam antibiotic
  • Agarose
    Polysaccharide, major component of Agar
  • Dimethylsulfoxid (DMSO)
    organic solvent
  • Acetic Acid
  • Ethylenediaminetetraacetic acid (EDTA)
  • Ethanol
  • TRIS
    buffer solution for enzymes
  • Nucleoside triphosphate
  • Trypton
  • Isopropanol
  • Isopropyl-β-D-thiogalactopyranosid (IPTG)
  • LB-medium
    used for growth of E.coli
  • Agar-Agar

Software

The following list of software was used in the team:

  • Unipro UGENE
    UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
  • Vector NTI (commercial)
    All our primers were designed using Vector NTI which is also used by our advisors.
  • BLAST
    BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
  • ApE
    The free and useful plasmid editor.
  • Google Drive
    Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).