Team:Tuebingen/NotebookAppendix

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= Appendix =
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''To complete this report we list all products and software used over the course of our project.''
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== Constructs ==
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<table class="wikitable">
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<tr><th>backbone pRS#</th><th>part #1</th><th>part #2</th><th>part #3</th></tr>
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<tr><td>313</td><td>Padh1</td><td>mPR ''Danio rerio''</td><td>Tadh1</td></tr>
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<tr><td>313</td><td>Padh1</td><td>mPR ''Xenopus laevis''</td><td>Tadh1</td></tr>
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<tr><td>315</td><td>Pfet3</td><td>rox1</td><td>Tadh1</td></tr>
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<tr><td>315</td><td>Pfet3</td><td>mig1</td><td>Tadh1</td></tr>
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<tr><td>316</td><td>Panb1</td><td>lacZ</td><td>Tadh1</td></tr>
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<tr><td>316</td><td>Panb1</td><td>luciferase</td><td>Tadh1</td></tr>
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<tr><td>316</td><td>Psuc2</td><td>lacZ</td><td>Tadh1</td></tr>
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<tr><td>316</td><td>Psuc2</td><td>luciferase</td><td>Tadh1</td></tr>
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</table>
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== Chemicals ==
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We needed the following chemicals:
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* Ampicillin <br /> beta-lactam antibiotic
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* Agarose <br /> Polysaccharide, major component of Agar
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* Dimethylsulfoxid (DMSO) <br /> organic solvent
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* Acetic Acid
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* Ethylenediaminetetraacetic acid (EDTA)
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* Ethanol
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* TRIS <br /> buffer solution for enzymes
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* Nucleoside triphosphate
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* Trypton
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* Isopropanol
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* Isopropyl-β-D-thiogalactopyranosid (IPTG)
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* LB-medium <br /> used for growth of E.coli
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* Agar-Agar
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== Software ==
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The following list of software was used in the team:
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* [http://ugene.unipro.ru/ Unipro UGENE] <br/> UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
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* [http://de-de.invitrogen.com/site/de/de/home/Products-and-Services/Applications/Cloning/vector-nti-software.html Vector NTI] (commercial) <br/> All our primers were designed using Vector NTI which is also used by our advisors.
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* [http://blast.ncbi.nlm.nih.gov/Blast.cgi BLAST] <br/> BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
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* [http://biologylabs.utah.edu/jorgensen/wayned/ape/ ApE] <br/> The free and useful plasmid editor.
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* [http://drive.google.com Google Drive] <br/> Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).

Latest revision as of 12:53, 26 September 2012



Contents

Appendix

To complete this report we list all products and software used over the course of our project.

Constructs

backbone pRS#part #1part #2part #3
313Padh1mPR Danio rerioTadh1
313Padh1mPR Xenopus laevisTadh1
315Pfet3rox1Tadh1
315Pfet3mig1Tadh1
316Panb1lacZTadh1
316Panb1luciferaseTadh1
316Psuc2lacZTadh1
316Psuc2luciferaseTadh1

Chemicals

We needed the following chemicals:

  • Ampicillin
    beta-lactam antibiotic
  • Agarose
    Polysaccharide, major component of Agar
  • Dimethylsulfoxid (DMSO)
    organic solvent
  • Acetic Acid
  • Ethylenediaminetetraacetic acid (EDTA)
  • Ethanol
  • TRIS
    buffer solution for enzymes
  • Nucleoside triphosphate
  • Trypton
  • Isopropanol
  • Isopropyl-β-D-thiogalactopyranosid (IPTG)
  • LB-medium
    used for growth of E.coli
  • Agar-Agar

Software

The following list of software was used in the team:

  • Unipro UGENE
    UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
  • Vector NTI (commercial)
    All our primers were designed using Vector NTI which is also used by our advisors.
  • BLAST
    BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
  • ApE
    The free and useful plasmid editor.
  • Google Drive
    Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).
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