Team:Tuebingen/NotebookAppendix

From 2012.igem.org

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(Kits and Protocols)
(Appendix)
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''To complete this report we list all products and software used over the course of our project.''
''To complete this report we list all products and software used over the course of our project.''
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=== Constructs ===
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<!--
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pRS mit jeweiligen Inserts
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=== Protocols ===
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* RV
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* Ligation
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* Transformation
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* komp. Zellen
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* PCR mit Temp.
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* Genaxxon PCR DNA Purification Mini Prep
* Genaxxon PCR DNA Purification Mini Prep
* Qiagen Plasmid Midi
* Qiagen Plasmid Midi
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=== Chemicals ===
=== Chemicals ===
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* LB-medium <br /> used for growth of E.coli
* LB-medium <br /> used for growth of E.coli
* Agar-Agar
* Agar-Agar
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=== Software ===
=== Software ===

Revision as of 09:38, 13 September 2012





Contents

Appendix

To complete this report we list all products and software used over the course of our project.

Constructs

Protocols

Kits

  • Genaxxon Plasmid DNA Purification Mini Prep
  • Genaxxon Gel Extraction Mini Prep
  • Genaxxon PCR DNA Purification Mini Prep
  • Qiagen Plasmid Midi


Chemicals

We needed the following chemicals:

  • Ampicillin
    beta-lactam antibiotic
  • Agarose
    Polysaccharide, major component of Agar
  • Dimethylsulfoxid (DMSO)
    organic solvent
  • Acetic Acid
  • Ethylenediaminetetraacetic acid (EDTA)
  • Ethanol
  • TRIS
    buffer solution for enzymes
  • Nucleoside triphosphate
  • Trypton
  • Isopropanol
  • Isopropyl-β-D-thiogalactopyranosid (IPTG)
  • LB-medium
    used for growth of E.coli
  • Agar-Agar


Software

The following list of software was used in the team:

  • Unipro UGENE
    UGENE is a free open-source cross-platform bioinformatics software. We used it to construct and annotate all needed sequences, search for restriction sites and visualization.
  • Vector NTI (commercial)
    All our primers were designed using Vector NTI which is also used by our advisors.
  • BLAST
    BLAST was our main sequence search tool. It was quite useful for controlling sequence results.
  • Google Drive
    Google Drive was used for our documentation and all of our collaborative work (papers, poster, images).