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Team:Tsinghua
From 2012.igem.org
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Revision as of 08:44, 23 September 2012
Domino is a popular game all over the world with very simple rules. When several dominoes are placed in a long line and are close enough, they could be knocked over one by one. The first toppled domino would topple the second one, and then the third one, etc... As a result, all the dominoes fall.
The easiest way of playing domino is to knock over a string of tiles by just pushing the first tile. Of course there are harder methods, for example, with the different design of placing dominoes, one could achieve different ways for tiles of being knocked down. Finally, with the combination of sophisticated design, beautiful and complex patterns would appear.
This simple and popular game inspires us greatly and we decided to draw an analogy to domino game by using E. coli. We use E. coli as tiles, and imitate the falling of domino by using different fluorescent proteins. Finally we need a signal to trigger the process and we decide to adopt AHL.
Our goal is that by adding AHL to modified E. coli at specific place, XFP would express and the expression of color could expand and spread, just like the falling down of dominoes. With our design, various approaches of falling down are achievable and if possible, sophisticated patterns could be obtained as well.
