Team:Trieste/parts/8

From 2012.igem.org

(Difference between revisions)
Line 30: Line 30:
</br>  
</br>  
<h2>Looking forward</h2>
<h2>Looking forward</h2>
 +
<p> In the final system, the toxin should be under the control of the T5 Cumate Operator (BBa_K875001).
 +
</p>
</br>
</br>
<h3><a href="http://partsregistry.org/Part:BBa_K875008"target="_blank">Link to the Registry</a></h3>
<h3><a href="http://partsregistry.org/Part:BBa_K875008"target="_blank">Link to the Registry</a></h3>

Revision as of 16:51, 24 September 2012

BBa_K875008

More

Description

The Tse2 protein is a toxin component of a toxin-immunity system and arrests the growth of prokaryotic and eukaryotic cells when expressed intracellularly. In contrast, secreted Tse2 had no effect on eukaryotic cell.


Assembly


Results



M15 bacteria cells (containing the pREP4 plasmid coding for the Lac repressor) are transformed with this Biobrick that resides on pSB1C3 plasmid.

After an over night liquid culture (LB broth) the OD600 absorbance is recorded and the culture is diluted to 0,1 and the batch is splitted into 6 flask – 20ml each. Three of these are induced with IPTG 1mM and the others are not induced.

The OD 600 is then recorded each hour.

It is possible to observe that not induced cells growth up to OD 1, instead the induced ones do not growth during the time, demonstrating the inducible promoter and toxin efficiency.


Modelling


Looking forward

In the final system, the toxin should be under the control of the T5 Cumate Operator (BBa_K875001).


Link to the Registry


Università degli studi di Trieste ICGEB Illy Fondazione Cassa di Risparmio
iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012 iGEM 2012
HTML Hit Counter