Team:Trieste/notebook8

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Week 8

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Suicide System

We tried to ultimate the testing construct: we cloned the RBS_B0031-Tse2 toxin- TT_B0015 upstream the J23100 promoter-CymR-TT_B0015, with some issues.

Antibody

This week we used E. coli strain HB2151 to test pelB-SIP and pelB-scFv production. First, we transformed HB2151 with plasmids containing two versions of scFv. After 4 hour induction of recombinant bacterial cultures, we separated the periplasm content and then we proceeded with SDS-PAGE and Western blotting. On western blot, obtained this way, the bands corresponding to our proteins are well visible and aspecific signals are not present.

Chassis

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Team iGEM 2012

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Università degli studi di Trieste ICGEB Illy Fondazione Cassa di Risparmio
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