Project Overview

PHA is a kind of bio-plastic produced by microbes.It's standard extract can be got after being dissolved in chloroform. In this project, we will reproduce the rose come out in the lines of the famous drama'Romeo and Juliet'by the synthesis of PHA. PHA can be detected by Nile Red reagent by being dyed red, which looks exactly like rose.

Achievement

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What's PHA

Recently, polyhydroxyalkanoates (PHA) have received a lot of attention for their potential use as bioplastic. They are naturally occurring biopolymers – under low nitrogen, phosphorus, or oxygen conditions, many bacteria produce protein- and lipid-coated granules of PHA for energy storage, and these granules can then in turn be harvested in large quantities as bioplastics. Bioplastics not only help reduce the reliance on petroleum-based plastics, but also have a wide range of applications in the agricultural and medical fields because they are biodegradable and biocompatible. The most common PHA is poly(3-hydroxybutyrate), or poly(3HB), which is produced by the bacteria Cupriavidus necator. Three genes – phaA, phaB, and phaC – make up the phaCAB operon that synthesizes the enzymes required for formation of poly(3HB), which is not elastic enough for general plastic applications.

Experiment

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ASSAY