Team:Tokyo Tech/Parts

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PARTS

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Parts

For a brief overview of our main results, please have a look at our Main Results page. [Go to "HOME"]


Favorite Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
WBBa_K934001CompositephaC1-A-B1 [P(3HB) synthesis]Taku Nakayama4208
WBBa_K934012CompositePlas-LuxIToshiki Hashimoto755
WBBa_K934022CompositePlux-LasIToshiki Hashimoto798
Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
BBa_K934016CompositePlas-LacINaoki Tsukuda1332
WBBa_K934024RegulatoryPlux/tet hybrid promoterToshiki Hashimoto72
WBBa_K934025ReporterPlux/tet-GFPToshiki Hashimoto955
BBa_K934026CompositePlux-LacINaoki Tsukuda1304

Best new BioBrick part (natural): BBa_K934001

We constructed a part containing phaC1-A-B1 in Biobrick format (BBa_K934001). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).

Best new BioBrick part (engineered): BBa_K934022

We constructed BBa_K934022 by combining Plux promter (BBa_R0062) and LasI (BBa_K081016). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part (BBa_K934012), we succeeded in constructing a positive feedback system.

Best improved part(s): BBa_K934012, BBa_K934024

BBa_K934012: By constructing BBa_K934012, we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator (BBa_K266000) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Moreover, BBa_R0079, the lasI promoter used in BBa_K266000, was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI (BBa_K934012) by using an effective lasI promoter (BBa_K649000) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.

BBa_K934024: By constructing BBa_K934024, we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet hybrid promoter (BBa_K934024) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.

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