Team:Tokyo Tech/Parts

From 2012.igem.org

(Difference between revisions)
(Best improved part(s): BBa_K934012, BBa_K934024)
(Best new BioBrick part (engineered): BBa_K934022)
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We constructed a part containing phaC1-A-B1 in Biobrick format ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).
We constructed a part containing phaC1-A-B1 in Biobrick format ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).
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=Best new BioBrick part (engineered): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] =
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=Best new BioBrick part (engineered): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] (+BBa K934012 will be positive feedback system)=
We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.
We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.

Revision as of 03:32, 27 September 2012

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PARTS

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Parts

For a brief overview of our main results, please have a look at our Main Results page. [Go to "HOME"]


Favorite Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
WBBa_K934001CompositephaC1-A-B1 [P(3HB) synthesis]Taku Nakayama4208
WBBa_K934012CompositePlas-LuxIToshiki Hashimoto755
WBBa_K934022CompositePlux-LasIToshiki Hashimoto798
Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
BBa_K934016CompositePlas-LacINaoki Tsukuda1332
WBBa_K934024RegulatoryPlux/tet hybrid promoterToshiki Hashimoto72
WBBa_K934025ReporterPlux/tet-GFPToshiki Hashimoto955
BBa_K934026CompositePlux-LacINaoki Tsukuda1304

Best new BioBrick part (natural): BBa_K934001

We constructed a part containing phaC1-A-B1 in Biobrick format (BBa_K934001). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).

Best new BioBrick part (engineered): BBa_K934022 (+BBa K934012 will be positive feedback system)

We constructed BBa_K934022 by combining Plux promter (BBa_R0062) and LasI (BBa_K081016). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part (BBa_K934012), we succeeded in constructing a positive feedback system.

Best improved part(s): BBa_K934012, BBa_K934025

BBa_K934012: By constructing BBa_K934012, we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator (BBa_K266000) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Moreover, BBa_R0079, the lasI promoter used in BBa_K266000, was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI (BBa_K934012) by using an effective lasI promoter (BBa_K649000) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.

BBa_K934025: By constructing BBa_K934025, we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet hybrid promoter (BBa_K934025) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.

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