Team:Tokyo Tech/Parts

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__NOTOC__
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PARTS</div>
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=Parts=
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For a brief overview of our main results, please have a look at our Main Results page.  
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For a brief overview of our main results, please have a look at our Main Results page. [[https://2012.igem.org/Team:Tokyo_Tech Go to "HOME"]]
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Tokyo_Tech 2012 iGEM Team Parts Sandbox</div>
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Tokyo_Tech 2012 iGEM Team Parts</div>
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| align="center" style="background:#f0f0f0;"|'''Length'''
| align="center" style="background:#f0f0f0;"|'''Length'''
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|||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934016 BBa_K934016]||Composite||LasR/3OC12HSL complex dependent LacI generator||Naoki Tsukuda||1332
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|||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934016 BBa_K934016]||Composite||Plas-LacI||Naoki Tsukuda||1332
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|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024]||Regulatory||Plux/tet hybrid promoter||Toshiki Hashimoto||72
|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934024 BBa_K934024]||Regulatory||Plux/tet hybrid promoter||Toshiki Hashimoto||72
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|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]||Reporter||Plux/tet-GFP||Toshiki Hashimoto||955
|W||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]||Reporter||Plux/tet-GFP||Toshiki Hashimoto||955
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|||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934026 BBa_K934026]||Composite||LuxR/3OC6HSL complex dependent LacI generator||Naoki Tsukuda||1304
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|||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934026 BBa_K934026]||Composite||Plux-LacI||Naoki Tsukuda||1304
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=Best new BioBrick part (natural): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]=
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We constructed a part containing phaC1-A-B1 in Biobrick format ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934001 BBa_K934001]). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).
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=Best new BioBrick part (engineered): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022]=
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(+BBa_K934012 is positive feedback system)</div>
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We constructed [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934022 BBa_K934022] by combining Plux promter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_R0062 BBa_R0062]) and LasI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K081016 BBa_K081016]). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]), we succeeded in constructing a positive feedback system.
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=Best improved part(s): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]=
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]:
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By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012], we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000]) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied.
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Moreover, [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0079 BBa_R0079], the lasI promoter used in [http://partsregistry.org/wiki/index.php?title=Part:BBa_K266000 BBa_K266000], was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934012 BBa_K934012]) by using an effective lasI promoter ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K649000 BBa_K649000]) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.
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[http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]:
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By constructing [http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025], we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet-GFP ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K934025 BBa_K934025]) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.
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<groupparts>iGEM012 Tokyo_Tech</groupparts>
<groupparts>iGEM012 Tokyo_Tech</groupparts>

Latest revision as of 03:52, 27 September 2012

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Tokyotechlogo2012.png

PARTS

Parts

For a brief overview of our main results, please have a look at our Main Results page. [Go to "HOME"]


Favorite Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
WBBa_K934001CompositephaC1-A-B1 [P(3HB) synthesis]Taku Nakayama4208
WBBa_K934012CompositePlas-LuxIToshiki Hashimoto755
WBBa_K934022CompositePlux-LasIToshiki Hashimoto798
Tokyo_Tech 2012 iGEM Team Parts


Name Type Description Designer Length
BBa_K934016CompositePlas-LacINaoki Tsukuda1332
WBBa_K934024RegulatoryPlux/tet hybrid promoterToshiki Hashimoto72
WBBa_K934025ReporterPlux/tet-GFPToshiki Hashimoto955
BBa_K934026CompositePlux-LacINaoki Tsukuda1304

Best new BioBrick part (natural): BBa_K934001

We constructed a part containing phaC1-A-B1 in Biobrick format (BBa_K934001). This is the first Biobrick part that succeeded in synthesizing Polyhydroxyalkanoates (PHAs).

Best new BioBrick part (engineered): BBa_K934022

(+BBa_K934012 is positive feedback system)

We constructed BBa_K934022 by combining Plux promter (BBa_R0062) and LasI (BBa_K081016). This is the first Biobrick part that succeeded in 3OC6HSL dependent 3OC12HSL production. Moreover, by co-culturing the cells containing this part with the cells containing 3OC6HSL dependent 3OC12HSL producer part (BBa_K934012), we succeeded in constructing a positive feedback system.

Best improved part(s): BBa_K934012, BBa_K934025

BBa_K934012: By constructing BBa_K934012, we improved a 3OC12HSL-dependent LuxI generator part. Even though a 3OC12HSL-dependent LuxI generator (BBa_K266000) was constructed by IPN-UNAM-Mexico 2009 team, no experimental data has been supplied. Moreover, BBa_R0079, the lasI promoter used in BBa_K266000, was evaluated as an ineffective promoter in the Experience Page of the Registry. Thus, in this study, we constructed a new Plas-LuxI (BBa_K934012) by using an effective lasI promoter (BBa_K649000) and confirmed its activity. This result can be considered as an improvement of the 3OC12HSL-dependent LuxI generator part.

BBa_K934025: By constructing BBa_K934025, we improved a Plux/tet hybrid promoter part. Even though a Plux/tet hybrid promoter was constructed by USTC 2009 team, their experiment did not contain the inducer combination: 3OC6HSL(-) and aTc(+). Thus, data of Plux/tet hybrid promoter in Biobrick Registry was not sufficient. In this study, we constructed a new Plux/tet-GFP (BBa_K934025) and confirmed its activity under all four combinations. This result can be considered as an improvement of the Plux/tet hybrid promoter part.

<groupparts>iGEM012 Tokyo_Tech</groupparts>