Team:Tokyo Tech

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A Project over view

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A love story contains several processes. Two people fall in love and their love burning wildly. However, no forever exists in the world, in most occasions, love will eventually burn to only a pile of ashes of the last remaining wind drift away. In our project, we have recreated the story of "Romeo & Juliet" by Shakespeare vividly by two kinds of Escherichia coli. We aim to generate a circuit involving regulatory mechanism of
positive feedback
rather than commonly-used negative feedback to control the fate of E.coli by signaling between two types of E.coli. Besides, Rose represents love. We are the first iGEM group ever to synthesize
PHA
(a kind of bio-plastics) which to represent rose by using our new biobrick part.

Ⅰ cell-cell communication

"Romeo and Juliette" is the dramas by dramatist William Shakespeare of England. The stage is city Verona, Italy in the 14th century. Romeo met Juliette. Two persons fall to love instantly. In this project, we will recreate the love story of "Romeo & Juliette".

In this project, we will recreate the love story of "Romeo&Juliette".

Ⅰ-1 story

We make our cute E.coli play “Romeo and Juliet” which is one of Shakespeare’s most famous plays. In this project, we define the signal which E.coli gives out as the romantic feeling of Romeo and Juliet.

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The story which we reproduce is divided into four scenes.

(Scene 1) Romeo and Juliet encountered and they fell in love with each other.

(Scene 2) However, providence lane person, this period of pure love might never broom since they were born in two hostile families. In order to keep their relationship, Juliet was planning to pretend died by drinking the poison so that when she woke up there will be no obstacle between their great loves. She planned and she did.

(Scene 3) However, this new was not conveyed to Romeo in time. Romeo misunderstood Juliet’s death and committed suicide.

(Scene 4) When Juliet woke up, it was not a beautiful future but Romeo’s death that is waiting for her. Juliet felt so sad and she followed Romeo by stabbing herself with a dirk.

Desingn of our circuit for project.

Gene Circuit


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Ⅰ-2 Positive Feedback of cell-cell communication

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Other experiments for basis of positive feedback system

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Detailed characterization of Lux-Tet Hybrid promoter: Key part for band detect

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Ⅰ-3 Modeling

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Ⅱ Organic synthesis of PHA

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There is what of the famous scene of "Romeo and Juliette"

JULIET: O Romeo, Romeo! why are you Romeo? Deny your father and refuse your name; Or, if you will not, be but sworn my love, And I'll no longer be a Capulet.

ROMEO: Shall I hear more, or shall I speak at this?

JULIET: It's but your name that is my enemy; you are yourself, though not a Montague. What's Montague? it is nor hand, nor foot, Nor arm, nor face, nor any other part Belonging to a man. O, be some other name! What's in a name? that which we call a rose By any other name would smell as sweet.

we will recreate the rose come out in the lines of the famous drama'Romeo and Juliet' by the synthesis of PHA.

Ⅲ Significance in SynBio

cell-cell communication

ecosystem

Organic synthesis of PHA

Water-repellent

Ⅳ Human Practice

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