Team:Tokyo-NoKoGen/Notebook/diary

From 2012.igem.org

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<BR>
 +
<BR>
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July<BR><BR>
 +
8-14<BR><BR>
 +
Meeting :<BR>
 +
lux team member: Jinhee LEE, Sumiya KAWAI, Miho NARITA, Tomomi YOKOYAMA, Mayumi NAKAMURA <BR>
 +
rhodopsin team member: Hiroto OKANO, Genki SAKAI, Hayato TSURUTA, Somin LEE
 +
<BR>
 +
<BR>
 +
 +
August<BR>
 +
5-11<BR><BR>
 +
Meeting : <BR><BR>
 +
Small session on gene experiments using iGEM biobrick.<BR><BR>
 +
Lux team :<BR>
 +
Design primers to clone lux operon from photobacterim phosphorium genome DNA.
 +
<BR>
 +
<BR>
 +
 +
19-25<BR><BR>
 +
Rhodopsin team :<BR>
 +
construction of blue light sensor.<BR>
 +
Pconst.(H)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-DT<BR>
 +
sequence analysis of constructed blue light sensor biobrick.<BR><BR>
 +
Meeting : <BR>
 +
lux team and rhodopsin team made small presentation about experiments.
 +
 +
<BR><BR>
 +
 +
26-9/1<BR><BR>
 +
Rhodopsin team :<BR>
 +
construction of ⊿EnvZ competent cell<BR><BR>
 +
Meeting :<BR>
 +
Our project title and team character was desided! <BR>
 +
“Coli express for long distance communication”<BR><BR>
 +
Lux team :<BR>
 +
lux operon was cloned from photobacterim phosphorium genome DNA. Most sequence was confirmed by sequence analysis.
 +
 +
<BR><BR>
 +
 +
2-8<BR><BR>
 +
Meeting :<BR>
 +
lux team and rhodopsin team made small presentation about experiments.<BR><BR>
 +
Rhodopsin team :<BR>
 +
insertion of PompR(+)-RBS-GFP-DT under blue light sensor biobrick.(BBa~)<BR>
 +
plasmid extraction of blue light sensor + GFP and sequence analysis.
 +
 +
<BR><BR>
 +
 +
9-15<BR><BR>
 +
Lux team : <BR>
 +
lux bio-brick (pSB1C3-PBAD-RBS-lux operon-DT) was constructed! E. coli TOP10 was transformed with this plasmid.<BR>
 +
Evaluation of lux bio-brick. Luminescence was measured using plate reader. We could see luminescence in dark room.<BR>
 +
Design primers to clone rib operon from E.coli genome DNA<BR>
 +
lux bio-brick that constitutive expression of lux gene was constructed. We expected more luminescence using strong constitutive expression promoter.<BR>
 +
<BR>
 +
Rhodopsin team :<BR>
 +
evaluation of blue light sensor.<BR>
 +
After preculture, transformants were culture under blue light or dark condition.<BR>
 +
But cells were not growth well.<BR><BR>
 +
Meeting :<BR>
 +
Our team T-shirt design idea has been completed.<BR>
 +
 +
<BR><BR>
 +
 +
16-22<BR><BR>
 +
Lux team :<BR>
 +
rib operon was cloned fromE.coli genome DNA. Most sequence was confirmed by sequence analysis<BR>
 +
Evaluation of color change biobrick.<BR><BR>
 +
Meeting :<BR><BR>
 +
Presentation team <BR>
 +
Poster session team<BR>
 +
We began to design poster and slide.<BR>
 +
 +
<BR><BR>
 +
 +
23-29<BR><BR>
 +
Rhodopsin team : <BR>
 +
Reevaluation of blue light sensor.<BR>
 +
After preculture, transformants were culture under white light or dark condition.<BR><BR>
 +
Lux team :<BR>
 +
evaluation of fast luminescence biobrick.<BR><BR>

Revision as of 06:40, 26 September 2012



July

8-14

Meeting :
lux team member: Jinhee LEE, Sumiya KAWAI, Miho NARITA, Tomomi YOKOYAMA, Mayumi NAKAMURA
rhodopsin team member: Hiroto OKANO, Genki SAKAI, Hayato TSURUTA, Somin LEE

August
5-11

Meeting :

Small session on gene experiments using iGEM biobrick.

Lux team :
Design primers to clone lux operon from photobacterim phosphorium genome DNA.

19-25

Rhodopsin team :
construction of blue light sensor.
Pconst.(H)-RBS-NpSRII-9a.a.linker_NpHtrII-EnvZ-DT
sequence analysis of constructed blue light sensor biobrick.

Meeting :
lux team and rhodopsin team made small presentation about experiments.

26-9/1

Rhodopsin team :
construction of ⊿EnvZ competent cell

Meeting :
Our project title and team character was desided!
“Coli express for long distance communication”

Lux team :
lux operon was cloned from photobacterim phosphorium genome DNA. Most sequence was confirmed by sequence analysis.

2-8

Meeting :
lux team and rhodopsin team made small presentation about experiments.

Rhodopsin team :
insertion of PompR(+)-RBS-GFP-DT under blue light sensor biobrick.(BBa~)
plasmid extraction of blue light sensor + GFP and sequence analysis.

9-15

Lux team :
lux bio-brick (pSB1C3-PBAD-RBS-lux operon-DT) was constructed! E. coli TOP10 was transformed with this plasmid.
Evaluation of lux bio-brick. Luminescence was measured using plate reader. We could see luminescence in dark room.
Design primers to clone rib operon from E.coli genome DNA
lux bio-brick that constitutive expression of lux gene was constructed. We expected more luminescence using strong constitutive expression promoter.

Rhodopsin team :
evaluation of blue light sensor.
After preculture, transformants were culture under blue light or dark condition.
But cells were not growth well.

Meeting :
Our team T-shirt design idea has been completed.


16-22

Lux team :
rib operon was cloned fromE.coli genome DNA. Most sequence was confirmed by sequence analysis
Evaluation of color change biobrick.

Meeting :

Presentation team
Poster session team
We began to design poster and slide.


23-29

Rhodopsin team :
Reevaluation of blue light sensor.
After preculture, transformants were culture under white light or dark condition.

Lux team :
evaluation of fast luminescence biobrick.

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