Team:Tianjin/Data

From 2012.igem.org

(Difference between revisions)
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=BioBrick=
=BioBrick=
===Favorite Tianjin 2012 iGEM Team Parts===
===Favorite Tianjin 2012 iGEM Team Parts===
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[[file:Tianjin_BB1.PNG]]
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<html>
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<tr>
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<td>1</td>
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<td>Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821001">BBa_K821001</a></td>
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<td>Reporter</td>
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<td>RFP Reporter with o-RBS</td>
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<td>Qinfeng Wu, Bin Jia</td>
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<td>1059</td>
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</tr>
 +
 
 +
<tr>
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<td>2</td>
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<td>Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821002">BBa_K821002</a></td>
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<td>Coding</td>
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<td>Orthogonal 16S DNA sequence from rrnB</td>
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<td>Qinfeng Wu, Bin Jia</td>
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<td>1894</td>
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</tr>
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 +
<tr>
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<td>3</td>
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<td>Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821012">BBa_K821012</a></td>
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<td>Generator</td>
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<td>PBad(promoter)+orthogonal 16S RNA</td>
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<td>Jinlai Zhang, Dongchang Qin</td>
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<td>3226</td>
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</tr>
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</html>
===Tianjin 2012 iGEM Team Parts Sandbox===
===Tianjin 2012 iGEM Team Parts Sandbox===
-
[[file:Tianjin_BB2.PNG]]
+
<html>
 +
<tr>
 +
<td>1</td>
 +
<td>Working</td>
 +
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
 +
<td>Coding</td>
 +
<td>16S DNA sequence from rrnB</td>
 +
<td>Jinlai Zhang, Dongchang Qin</td>
 +
<td>1894</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>2</td>
 +
<td>Working</td>
 +
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821004">BBa_K821004</a></td>
 +
<td>Regulatory</td>
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<td>PBad Promoter </td>
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<td>Jinlai Zhang, Dongchang Qin</td>
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<td>1324</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>3</td>
 +
<td>Working</td>
 +
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821005">BBa_K821005</a></td>
 +
<td>Reporter</td>
 +
<td>CmR label from pKD3</td>
 +
<td>Qinfeng Wu, Bin Jia</td>
 +
<td>1014</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>4</td>
 +
<td>Working</td>
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<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821006">BBa_K821006</a></td>
 +
<td>Project</td>
 +
<td>KanR label from pKD3</td>
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<td>Jinlai Zhang, Dongchang Qin</td>
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<td>1304</td>
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</tr>
 +
 
 +
<tr>
 +
<td>5</td>
 +
<td>Working</td>
 +
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821007">BBa_K821007</a></td>
 +
<td>Composite</td>
 +
<td>PL(promoter)+orthogonal 16S RNA</td>
 +
<td>Qinfeng Wu, Bin Jia</td>
 +
<td>1957</td>
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</tr>
 +
 
 +
<tr>
 +
<td>6</td>
 +
<td>Working</td>
 +
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821008">BBa_K821008</a></td>
 +
<td>Composite</td>
 +
<td>PLtet+orthogonal 16S RNA</td>
 +
<td>Jinlai Zhang, Dongchang Qin</td>
 +
<td>1956</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>7</td>
 +
<td>Working</td>
 +
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821009">BBa_K821009</a></td>
 +
<td>Composite</td>
 +
<td>Orthogonal RBS 1</td>
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<td>Qinfeng Wu, Bin Jia</td>
 +
<td>6</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>8</td>
 +
<td>Working</td>
 +
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
 +
<td>Coding</td>
 +
<td>16S DNA sequence from rrnB</td>
 +
<td>Jinlai Zhang, Dongchang Qin</td>
 +
<td>1894</td>
 +
</tr>
 +
 
 +
<tr>
 +
<td>1</td>
 +
<td>Working</td>
 +
<td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K821003">BBa_K821003</a></td>
 +
<td>Coding</td>
 +
<td>16S DNA sequence from rrnB</td>
 +
<td>Jinlai Zhang, Dongchang Qin</td>
 +
<td>1894</td>
 +
</tr>
 +
</html>
=Agarose gel electrophoresis=
=Agarose gel electrophoresis=

Revision as of 15:21, 26 September 2012

Contents

BioBrick

Favorite Tianjin 2012 iGEM Team Parts

1 Working BBa_K821001 Reporter RFP Reporter with o-RBS Qinfeng Wu, Bin Jia 1059 2 Working BBa_K821002 Coding Orthogonal 16S DNA sequence from rrnB Qinfeng Wu, Bin Jia 1894 3 Working BBa_K821012 Generator PBad(promoter)+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 3226

Tianjin 2012 iGEM Team Parts Sandbox

1 Working BBa_K821003 Coding 16S DNA sequence from rrnB Jinlai Zhang, Dongchang Qin 1894 2 Working BBa_K821004 Regulatory PBad Promoter Jinlai Zhang, Dongchang Qin 1324 3 Working BBa_K821005 Reporter CmR label from pKD3 Qinfeng Wu, Bin Jia 1014 4 Working BBa_K821006 Project KanR label from pKD3 Jinlai Zhang, Dongchang Qin 1304 5 Working BBa_K821007 Composite PL(promoter)+orthogonal 16S RNA Qinfeng Wu, Bin Jia 1957 6 Working BBa_K821008 Composite PLtet+orthogonal 16S RNA Jinlai Zhang, Dongchang Qin 1956 7 Working BBa_K821009 Composite Orthogonal RBS 1 Qinfeng Wu, Bin Jia 6 8 Working BBa_K821003 Coding 16S DNA sequence from rrnB Jinlai Zhang, Dongchang Qin 1894 1 Working BBa_K821003 Coding 16S DNA sequence from rrnB Jinlai Zhang, Dongchang Qin 1894

Agarose gel electrophoresis

7.14 t7 rbs' crtE
7.31 16s' RFP-GFP AMP


8.03 pSB1C3 K3 Digestion
8.16 16s' mutate to remove EcoRI


8.17 16S' pSB1C3
8.17 Mutate the RBS of Amp using PYES2


8.17 Yeast Assembly using pSB3C5R & pSB3K3
8.18 pBad-16S' remove EcorI, PSB1C3


9.2 6I 6G Gel extraction and verification
9.2 6I,6G P16S Gel extraction and verification


9.2 P16S digestion
9.16 yeast cPCR 1


9.16 yeast cPCR 2
81 16s GFP & RFP



</div>
Retrieved from "http://2012.igem.org/Team:Tianjin/Data"