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Contents 1 The general idea 2 The inducers, promoters and bacterial strain 3 The fluorescent proteins 4 The RNA polymerases and promoters 5 The phage

The general idea

Figure 1 presents the basic strategy for our three inputs AND gate. Each one of the inducers can be an environmental signal, to which the phage will respond. In the presence of all the inputs, the phage will be capable to resume its lytic cycle. The assembly of the lytic phage, is our circuit's output.

The inducers, promoters and bacterial strain

The fluorescent proteins

The RNA polymerases and promoters

The phage