Team:Technion/Attributions

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Attributions

During our project we have used parts that were given to us by different labs or were taken from the registry/distribution kit.

Donated parts

Ann K. Ganesan - WT T7 RNAP
Changwon Kang - WT SP6 RNAP
Christopher A. Voight - T7*, T7*(T3), T7*(N4), T7*(K1F) RNAPs with suitable induced promoters: pT7, pT3, pK1F, pN4
Gallivan Lab (Gallivan Justin P., Lynch Sean A.) - Theophylline riboswitch and pTAC
Roee's Lab (Roee Amit) - Cerulean FP under control of pLac/Ara, mCitrin FP and mCherry under control of pTetO.

Parts from Distribution Kit


Name	Description
BBa_B0015	Double terminator
BBa_F2620	TetR controllable LuxR and lux pR genes
BBa_I0462**	LuxR protein generator
BBa_I13522**	GFP under pTetO control
BBa_J06504**	mCherry FP
BBa_J06702	mCherry, bacterial with RBS and forward terminator
BBa_J23119	Constitutive promoter
BBa_R0062	Promoter (luxR & HSL regulated -- lux pR)
pSB1C3	High copy BioBrick assembly plasmid
pSB3C5	Low to medium copy BioBrick standard vector

Notes: Parts followed by ** are parts that haven't worked because distribution kit contained some other DNA in the well where the part should be.

We have submitted 25 BioBricks to the registry. Due to lack of time we haven't managed to put all the parts inside the shipping plasmid.

We have managed to successfully clone 4 out of 8 parts that phage's genome had been divided into.

We have creatednew reporter system to test our YES gates and hope that it will become standard system for testing other parts as well. We used it to characterize T7 RNAP activity, the results can be seen here.