Team:Technion/Attributions

From 2012.igem.org

(Difference between revisions)
(Attributions)
(General)
Line 33: Line 33:
|}
|}
===General===
===General===
 +
* The image of the bacteriophage used for the creation of our banner was made by Jonathan Heras from Equinox Graphics Ltd. and was found on [http://www.sciencemag.org/content/331/6019/848.full#F3 science magazine 2010 visualization challenge]
==Contributions==
==Contributions==

Revision as of 16:09, 26 September 2012



Contents

Attributions

During our project we have used parts that were given to us by different labs or were taken from the registry/distribution kit.

Donated parts

  • Ann K. Ganesan - WT T7 RNAP
  • Changwon Kang - WT SP6 RNAP
  • Christopher A. Voight - T7*, T7*(T3), T7*(N4), T7*(K1F) RNAPs with suitable induced promoters: pT7, pT3, pK1F, pN4
  • Gallivan Lab (Gallivan Justin P., Lynch Sean A.) - Theophylline riboswitch and pTAC
  • Roee's Lab (Roee Amit) - Cerulean FP under control of pLac/Ara, mCitrin FP and mCherry under control of pTetO.

Parts from Distribution Kit

Name Description
BBa_B0015 Double terminator
BBa_F2620 TetR controllable LuxR and lux pR genes
BBa_I0462 LuxR protein generator
BBa_I13522 GFP under pTetO control
BBa_J06702 mCherry, bacterial with RBS and forward terminator
BBa_R0062 Promoter (luxR & HSL regulated -- lux pR)
pSB1C3 High copy BioBrick assembly plasmid
pSB3C5 Low to medium copy BioBrick standard vector

General

Contributions

  • We have submitted DNA for 25 BioBricks to the registry. Due to lack of time we haven't managed to put all the parts inside the shipping plasmid.
  • We have managed to successfully clone 4 out of 8 lambda phage fragments that the phage's genome has been divided into.
  • We have created new reporter system to test our YES gates and hope that it will become standard system for testing other parts as well. We used it to characterize T7 RNAP activity, the results can be seen here.
  • We have managed to combine a theophylline riboswitch with T7, T7*(T3), T7*(K1F), T7*(N4) by using fusion PCR under the control of a tac promoter, more details can be seen at RS section. The riboswitch with T7 and T7*(N4) were cloned into the shipping plasmid and submitted as BioBricks. Due to lack of time we hadn't transferred RS+RNAP constructs under pLux control, as was planned at the beginning.
  • We have produced a working YES gate #1 with T7* RNAP.
  • We have added MCS to pSB1C3 plasmid backbone. This backbone is very convenient to work with, some of our parts had the same restriction sites as standard prefix and suffix found in the backbone, so we couldn't insert them into the pSB1C3 plasmid. Addition of MCS helps to circumvent this problem.
  • We entered experience for the following existing BioBricks: pSB3C5 and BBa_I0462.