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Team:Technion/Attributions
From 2012.igem.org
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|[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]|| TetR controllable LuxR and lux pR genes | |[http://partsregistry.org/Part:BBa_F2620 BBa_F2620]|| TetR controllable LuxR and lux pR genes | ||
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| - | |[http://partsregistry.org/Part:BBa_I0462 BBa_I0462] | + | |[http://partsregistry.org/Part:BBa_I0462 BBa_I0462]|| LuxR protein generator |
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| - | |[http://partsregistry.org/Part:BBa_I13522 BBa_I13522] | + | |[http://partsregistry.org/Part:BBa_I13522 BBa_I13522]|| GFP under pTetO control |
| - | + | ||
| - | + | ||
|+ | |+ | ||
|[http://partsregistry.org/Part:BBa_J06702 BBa_J06702]|| mCherry, bacterial with RBS and forward terminator | |[http://partsregistry.org/Part:BBa_J06702 BBa_J06702]|| mCherry, bacterial with RBS and forward terminator | ||
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|[http://partsregistry.org/Part:pSB3C5 pSB3C5]|| Low to medium copy BioBrick standard vector | |[http://partsregistry.org/Part:pSB3C5 pSB3C5]|| Low to medium copy BioBrick standard vector | ||
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==Contributions== | ==Contributions== | ||
Revision as of 15:05, 26 September 2012
- Home
- Team
- Project
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- Notebook
- Safety
- Attributions & Achievements
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- Sponsors & Thanks
Attributions
During our project we have used parts that were given to us by different labs or were taken from the registry/distribution kit.
Donated parts
- Ann K. Ganesan - WT T7 RNAP
- Changwon Kang - WT SP6 RNAP
- Christopher A. Voight - T7*, T7*(T3), T7*(N4), T7*(K1F) RNAPs with suitable induced promoters: pT7, pT3, pK1F, pN4
- Gallivan Lab (Gallivan Justin P., Lynch Sean A.) - Theophylline riboswitch and pTAC
- Roee's Lab (Roee Amit) - Cerulean FP under control of pLac/Ara, mCitrin FP and mCherry under control of pTetO.
Parts from Distribution Kit
| Name | Description | BBa_B0015 | Double terminator | BBa_F2620 | TetR controllable LuxR and lux pR genes | BBa_I0462 | LuxR protein generator | BBa_I13522 | GFP under pTetO control | BBa_J06702 | mCherry, bacterial with RBS and forward terminator | BBa_J23119 | Constitutive promoter | BBa_R0062 | Promoter (luxR & HSL regulated -- lux pR) | pSB1C3 | High copy BioBrick assembly plasmid | pSB3C5 | Low to medium copy BioBrick standard vector |
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Contributions
- We have submitted 25 BioBricks to the registry. Due to lack of time we haven't managed to put all the parts inside the shipping plasmid.
- We have managed to successfully clone 4 out of 8 parts that phage's genome had been divided into.
- We have created new reporter system to test our YES gates and hope that it will become standard system for testing other parts as well. We used it to characterize T7 RNAP activity, the results can be seen here.
- We have managed to combine RS with T7, T7*(T3), T7*(K1F), T7*(N4) by using fusion PCR and put the resulting construct under control of pLac, more details can be seen at RS section. Due to lack of time we hadn't transferred RS+RNAP constructs under pLux control, as was planned at the beginning.
- We have produced a working YES gate #1 with T7* RNAP.
- We have added MCS to pSB1C3 plasmid backbone. This backbone is very convenient to work with, however some of our parts had the same restriction sites as standard prefix and suffix found in the backbone, so we couldn't insert them into the pSB1C3 plasmid. Addition of MCS helps to circumvent this problem.
