From 2012.igem.org
Team:Technion/8_July_2012
Ilya
Inbal
Today I did transformation of the following BioBricks: BBa_J23119 and BBa_I0462.
First, I added 10 μL DW to their locations in the plate kit (plate No. 1).
Second, I did the transformation of those BioBricks to component cells. The medium for the recovery was SOB (800 μL).
Then, after shaking the cells for an hour at 37 degrees, I plated the cells on LB+Amp plates twice for each BioBrick, different volumes: 10μL and 100μL (4 plates in total).
After that the plates were incubated overnight in the incubator 37 degrees.
Asaf
Hila
Lior
Noa
Evgeni
Shahar
Rachel
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