From 2012.igem.org
Team:Technion/6_June_2012
Ilya
Yesterday Inbal and Asaf set up the starters and we made 80% glycerol for our stock.
Today Inbal and I minipreped the plasmids from the 3 transformations, measured their concentration and made glycerol stocks from the bacteria.
The concentrations of the plasmids are:
pUC19+12.1: 120 ng/ul
pSB1A2+R0062: 102 ng/ul
pSB1A2+J06702: 320 ng/ul
We have 28 ul of each of the plasmid solutions.
We put the plasmids in -20 degrees and the glycerol stocks in -80 degrees.
Tomorrow we will start the PCR steps for the fusion PCR.
Inbal
Asaf
Hila
Lior
Noa
Evgeni
Shahar
Rachel
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