Team:Technion/6 June 2012
From 2012.igem.org
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Ilya
Yesterday Inbal and Asaf set up the starters and we made 80% glycerol for our stock. Today Inbal and I minipreped the plasmids from the 3 transformations, measured their concentration and made glycerol stocks from the bacteria. The concentrations of the plasmids are: pUC19+12.1: 120 ng/ul pSB1A2+R0062: 102 ng/ul pSB1A2+J06702: 320 ng/ul We have 28 ul of each of the plasmid solutions. We put the plasmids in -20 degrees and the glycerol stocks in -80 degrees. Tomorrow we will start the PCR steps for the fusion PCR.