Team:Technion/30 July 2012

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Team:Technion/30_July_2012
March
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April
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30
May
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June
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July
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August
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September
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October
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November
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Ilya

- Glycerol stock of R0062+RS+mCh(clones #7 and #8) and of J23119+RS+mCh (clones #11 and #12). Freezer positions are in the excel file.
- Minipreped the above clones (7,8,11,12). Concentrations are (ng/ul): 7a - 162, 7b - 197, 8a - 153, 8b - 191, 11a - 155, 11b - 146, 12a - 176, 12b - 165.
- Restriction of ~3ug I0462 (luxR) with XbaI and PstI. Restriction of R0062+RS+mCh #7 and #8 (~1.5 ug each) with SpeI and PstI.
- Prepared a gel for gel purification.
- Prepared clones #7, #8, #11 and #12 for sequencing.

Inbal

Asaf

Hila

Transformation to competent cells of:
pTet + CFP (BBa_I13600)
pTet + GFP (BBa_I13522)

Lior

Noa

Evgeni

Shahar

Rachel

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