Team:Technion/30 August 2012

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Revision as of 14:40, 5 September 2012

Team:Technion/30_August_2012

March
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Ilya

Inbal

Asaf

I tried again the PCR of the 3 polymerase genes that failed the previues PCR (T7*,N4,K1F), now with Tm of 66C.
I tried this Tm because I got results with this Tm before (29.8 polymerase gel 2). This time I got it right,
I got the 2.7 kb bands I was expecting (althouge N4 is rather week).

Hila

Lior

Noa

olony PCR for XyIE bacteria described on 28.
Positive results for all strains!! yeah :)

Evgeni

- Transformation of TOP10 RB cells with pPROLar+Bba_015 ligated part
- Plating of transformed cells on Kanamycin plates - 100 ul and rest

Shahar & Rachel

We tried to do the failed PCR's from yesterday, but with lower temprature. For N4 (with A2 & plac/ara primers)we tried with temprature gradient.

@@ Line 17: / Line 17: @@
 ==Noa==
+olony PCR for XyIE bacteria described on 28. <br>
+Positive results for all strains!! yeah :)
 ==Evgeni==