From 2012.igem.org
Team:Technion/29_August_2012
Ilya
- Glycerol stock of pSB1C3+Fus2del (freezer position G2) and pSB3C5+Fus2del (freezer position G3).
- Plate reader assay for pSB1C3+Fus2 and pSB3C5+Fus2 with the positive (pSB1C3+Fus2del and pSB3C5+Fus2del) and negative (pSB1C3+MCS) controls.
Inbal
Asaf
-I checked the results of yesterday's PCR for extracting the polymerase genes on gel agarose,
and got two bands in T7*,T3 and K1f: one in 2kb and one near 3kb, and no bands in SP6 and N4.
I realized that I put an incorrect elongation time and this can explain the double band.
The Sp6 gene was probably defected.
-I ran another PCR reaction for the polymerase genes. This time with the right elongation time, but with Tm of 60C.
I didn't got any bands with the exception of T3.
Hila
Lior
Noa
Colony PCR for ALP bacteria described yesterday.
Positive results for: ALP+ T3 , N4.
Evgeni
- Overnight ligation of pPROLar backbone with Bba_015
Rachel & Shahar
PCR to Chris's Rnap with the new primers. The PCR with plac/ARA primer and T3, K1F & T7* templates, were successful.
The PCR with A@ primer and T3, K1F, N4 & T7* templates, failed.
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