Team:Technion/26 August 2012

From 2012.igem.org

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==Lior==
==Lior==
PCR purification of cutting products (pT3 pN4 pK1F pSp6 and xyIE). <br>
PCR purification of cutting products (pT3 pN4 pK1F pSp6 and xyIE). <br>
-
Ligation of p- plasmids with inserts + control: without insert for all plasmids.
+
Ligation of p- plasmids with inserts + control: without insert for all plasmids. <br>
Starters for done pT7 - inserts.
Starters for done pT7 - inserts.

Revision as of 12:52, 27 August 2012



Team:Technion/26_August_2012
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Ilya

- Starters of tac+RBS+mCh in pSB1C3 and in pSB3C5, R0062+RBS+mCh in pSB1C3 and in pSB3C5 for PacI restriction test tomorrow.

Inbal

- Ordered the new primers for T7 RNAP from chris. Also, I designed the final primers for the Gibson Assembly- hope that it will turn out good, Now I'm waiting for the results from the SP6 sequencing, before I will order the primers for Gibson. :)
- I digested the pTetO+GFP+ter (BBa_I13522) with EcoRI and PstI for 15 minutes at 37C, ran the products on 1% agarose gel for 30 minutes, The expected results were: 2 bands- one is 2000bp and the second is 940 bp approximately.
The actual results were completely not logical- I got bands at 1500bp and 3000bp, Also in the control lane there were number of bands (The control was the uncut plasmid). My conclusion for the this past two weeks, while working with BBa_I13522 is that something wrong with it. Tomorrow I will start working with a different BioBrick, one that contains pTetO and a reporting gene.

Asaf

Hila

Lior

PCR purification of cutting products (pT3 pN4 pK1F pSp6 and xyIE).
Ligation of p- plasmids with inserts + control: without insert for all plasmids.
Starters for done pT7 - inserts.

Noa

Evgeni

Shahar

Rachel