From 2012.igem.org
Team:Technion/23_August_2012
Ilya
Inbal
- I made today 2 new starters of the BioBrick BBa_I13522.
-Planing new primers for the T7 RNAP from chris.
- Sending to sequence the pSB1C3+SP6 plasmid (Conc: 78 ng/ul).
-Planing the primers for the Gibson assembly.
Asaf
Hila
The bacteria grew on the negative control (no insert) plates… :(
Colony PCR for:
- Six positive colonies (pSB1C3+MCS)
- Two negative control colonies (pSB1C3 without insert?!)
- Positive control (pSB1C3 + Fus_1)
- Negative control (no colony inserted)
1% gel for the PCR products.
I got five positive colonies (number 1-4, 6) with the wanted 344 bp band.
Colony number 5 showed a 1106 bp band (consider negative for the ligation) like the negative control colonies and the positive control for the experiment.
We think that the CIP enzyme was contaminated so the reaction was not as good as it should…
No bend appeared in the negative control strip.
Lior
Colony PCR for xyIE: we got 1/5.
PCR purification for pT3, pN4, pSp6, we got high concentrations- yeah :)
Cutting of plasmis and inserts with XhoI and XmaI.
Noa
Evgeni
Shahar
Rachel
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