Team:Technion/20 September 2012

From 2012.igem.org



Team:Technion/20_September_2012
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Ilya

Wiki, wiki, wiki...

Inbal

- The transformation worked! I got colonies on both transformation!(of T3 and also In1 with xyIE/ALP)- so I made starters for them.. The plates were stored at 4C.

Asaf

I transformed my lygated plasmid+inserts to Top 10 bacteria strain.

Hila

- Overnight ligation to the restriction products, into pSB1C3+MCS.
- PCR for all fragments with 100bp fragments overlap for the Gibson assembly tryout…
- Ladder experiment's for the BEST LADDER EVERRRR!!!

Lior

Preparing the shipping plasmids

Noa

Preparing the shipping plasmids

Evgeni

Shahar

PCR for all fragments with 100bp fragments overlap for the Gibson assembly tryout.

Rachel

4 miniprep for each RNAP(K1F, T3, N4)- at total 12 miniprep.