Team:Technion/19 August 2012
From 2012.igem.org
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Ilya
- All the white colony PCR colonies were positive on the gel. I picked one from each clone and put a starter.
Inbal
Today was pretty theoretical for me- I read and planned the Gibson assembly, Also I found out that it isn't piece of cake.
In addition, I measured the concentration of the mini preped pSB1C3+SP6 gene product: 105ng/ul.
Asaf
Hila
Lior
Noa
To do list:
1. PCR for Chris's plasmids: promoter plasmids (5 total)
2. a. PCR purification for PCR products of xyIE.
2. b. Plates for plating the ligated products- with XXX antibiotic.
3. PCR purification for PCR products of Chris's plasmids.
4. Restriction with XmaI + XhoI for:
- xyIE PCR products
- Chris's promoter plasmids (5 reactions total)
5. PCR purification for cut products of xyIE and Chris's plasmids
6. Ligation of xyIE and Chris's plasmids: 5 reactions total.
If there is time: PCR for alkaline phosphatase and running on Gel.