Team:Technion/19 August 2012

From 2012.igem.org



Team:Technion/19_August_2012
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Ilya

- All the white colony PCR colonies were positive on the gel. I picked one from each clone and put a starter.

Inbal

Today was pretty theoretical for me- I read and planned the Gibson assembly, Also I found out that it isn't piece of cake.
In addition, I measured the concentration of the mini preped pSB1C3+SP6 gene product: 105ng/ul.

Asaf

Hila

Lior

Noa

To do list:

1. PCR for Chris's plasmids: promoter plasmids (5 total)

2. a. PCR purification for PCR products of xyIE.
2. b. Plates for plating the ligated products- with XXX antibiotic.

3. PCR purification for PCR products of Chris's plasmids.

4. Restriction with XmaI + XhoI for:
- xyIE PCR products
- Chris's promoter plasmids (5 reactions total)

5. PCR purification for cut products of xyIE and Chris's plasmids

6. Ligation of xyIE and Chris's plasmids: 5 reactions total.

If there is time: PCR for alkaline phosphatase and running on Gel.

Evgeni

Shahar

Rachel