Team:Technion/19 August 2012
From 2012.igem.org
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1. PCR for Chris's plasmids: promoter plasmids (5 total) <br> | 1. PCR for Chris's plasmids: promoter plasmids (5 total) <br> | ||
- | 2. PCR purification for PCR products of xyIE. <br> | + | 2. a. PCR purification for PCR products of xyIE. <br> |
- | 2. | + | 2. b. Plates for plating the ligated products- with XXX antibiotic. <br> |
3. PCR purification for PCR products of Chris's plasmids. <br> | 3. PCR purification for PCR products of Chris's plasmids. <br> |
Revision as of 10:50, 18 August 2012
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Ilya
Inbal
Asaf
Hila
Lior
Noa
To do list:
1. PCR for Chris's plasmids: promoter plasmids (5 total)
2. a. PCR purification for PCR products of xyIE.
2. b. Plates for plating the ligated products- with XXX antibiotic.
3. PCR purification for PCR products of Chris's plasmids.
4. Restriction with XmaI + XhoI for:
- xyIE PCR products
- Chris's promoter plasmids (5 reactions total)
5. PCR purification for cut products of xyIE and Chris's plasmids
6. Ligation of xyIE and Chris's plasmids: 5 reactions total.
If there is time: PCR for alkaline phosphatase and running on Gel.