Team:Technion/10 July 2012
From 2012.igem.org
|
|
|
|
|
|
|
|
|
Ilya
Asaf and Inbal
We prepared 2 new PCR reactions for adding prefix and suffix to the RS+mCherry (f2).
In one we used GC buffer and in the other one HF buffer.
We ran the PCR products on gel and received a 850 bp band in all cases (HF/GC).
We cut the 850 bp bands from the gel and stored it in 4 °C for the night.
We made glycerol stocks of BBA-J23119 and I0462, but because it stayed outside for a couple of hours
we decided to prepare a new starter. We put the new starters in the shaker.
We opened the new miniprep kit (red box in the middle upper shelf) and prepared the solutions.
The resuspension solution is in the 4°C fridge.