Team:TU Munich/Project/Overview

From 2012.igem.org

Revision as of 19:30, 24 September 2012 by Fabian Froehlich (Talk | contribs)


Contents

Overview


Motivation

When genetically modified crops are used in food production a multitude of issues arise, ranging from contamination of unmodified species to increased use of agrochemicals (Benbrook 2009[1]).

In contrast, when the microorganisms utilized in food production are genetically modified instead of the crops, most of the problems can be alleviated.

Vector Design


50 - 70 words introduction



Explanations on the figure
Experimental results:

50-100 words on results

Regulation of Genexpression


50-100 words introduction

Constituvie promoters

50 - 70 words introduction



Experimental results:

50-100 words on results

Conclusion and outlook:

Ethanol-inducible promoter

50 - 70 words introduction



Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:

Light-switchable promoter

The idea behind a lightswitchable system is to create a gene expression system which can be induced and deactivated by light of a certain wavelengths.

This system is extremely attractive, as induction does not require the addition of a specific substance. This makes induction cheap, fast, precise and also compatible to the bavarian purity law.


File:TUM12 experiment overwiew light.png
Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:

Biosynthesis pathways


50-100 words introduction

Limonene

Limonene is a cyclic terpene and a major constituent of several citrus oils. D-Limonene has been used as a component of flavorings and fragrances. It is formed from geranyl pyrophosphate by limonene synthase.

We will produce the flavoring substance limonene by expressing limonene synthase in S. cerevisiae, which naturally synthesizes the educt geranyl pyrophosphate.


Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:

Thaumatin

Thaumatin is a natural α+β-protein which is synthesized by the katamfe plant (Thaumatococcus daniellii). It is said to be 2.000 to 100.000 times sweeter than sucrose on molar basis, but the sweetness builds slow and lasts long. It has been approved as a sweetener by the European Union (E957).

Our aim is to have S. cerevisiae secrete functional Thaumatin by expressing Preprothaumatin – a principle which has been proven by Edens et al. in 1984.(1)


Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:

Caffeine

50 - 70 words introduction



Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:

Xantohumol

50 - 70 words introduction



Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:

Genome integration


As we can't obey the letter of the German Purity Law (there is a zero tolerance policy concerning transgenic ingredients), we try our best to meet the spirit. Thus, it is unacceptable for us to work with antibiotics to keep up the selective environment. Since we can't work with auxotrophies in beer either, we have to make sure the yeasts don't get rid of the biobricks. The most promising way to accomplish a long lasting presence of our constructs is to achieve genome integration.



Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:

Brewing our SynBio Beer


50 - 70 words introduction



Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:

Businessplan for our TUM Brew


50 - 70 words introduction



Experimental results:

50-100 words on results

Conclusion and outlook:

Our RFC for Standardization of BioBrick part descriptions


50 - 70 words introduction



File:TUM12 experiment overwiew RFC.png
Explanations on the figure
Experimental results:

50-100 words on results

Conclusion and outlook:


References


  • [1] Synthesis and processing of the plant protein thaumatin in yeast, Luppo Edens, Isaäc Bom, Adrianus M. Ledeboer, Jan Maat, Marjolein Y. Toonen, Chris Visser, C. Theo Verrips. Cell Volume 37, Issue 2, June 1984, Pages 629–633
  • [2] C.L Miranda, J.F Stevens, V Ivanov, M McCall, B Frei, M.L Deinzer, D.R Buhler, Antioxidant and prooxidant actions of prenylated and nonprenylated chalcones and flavanones in vitro, J. Agric. Food Chem., 48 (2000), pp. 3876–3884
  • [3] Insertion of Coffein, Römpp Online. Version 3.19
  • [4] Use of the KlADH4 Promoter for Ethanol-Dependent Production of Recombinant Human Serum Albumin in Kluyveromyces lactis. Michele Saliola, Cristina Mazzoni, Nicola Solimando, Alessandra Crisà, Claudio Falcone, Gerard Jung, and Reinhard Fleer. Appl Environ Microbiol. 1999 January; 65(1): 53–60.
  • [5] Development of a genetic switch based on a photosensitive protein signals the beginning of light-based biological engineering. Andrew R. Mendelsohn Nature Biotechnology, Vol. 20, October 2002, Pages 985-987
  • [6] Molecular analysis of UAS, a cis element cotaining stress response elements responsible for ethanol induction in the KlADH4 of Kluyveromyces lactis. Cristina Mazzoni, Francesca Santori, Michele Saliola and Claudio Falcone Research in Microbiology, Vol. 151, 2000, Pages 19-28