"
Team:TU Darmstadt/Materials/NEB buffers
From 2012.igem.org
(Difference between revisions)
(Created page with "<html> <link rel="stylesheet" href="https://2012.igem.org/wiki/index.php?title=Team:TU_Darmstadt/css&action=raw&ctype=text/css" type="text/css" /> <div id="TUD"> <div id="...") |
|||
| Line 27: | Line 27: | ||
<li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | <li><a href="/Team:TU_Darmstadt/Safety" title="Safety">Safety</a></li> | ||
<li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | <li><a href="/Team:TU_Darmstadt/Downloads" title="Downloads">Downloads</a></li></ul></li> | ||
| - | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a | + | <li><a href="/Team:TU_Darmstadt/Human_Practice" title="Human Practice">Human Practice</a></li> |
| - | + | ||
| - | + | ||
| - | + | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Sponsors</a><ul> | ||
<li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | <li><a href="/Team:TU_Darmstadt/Sponsors" title="Sponsors">Overview</a></li> | ||
Latest revision as of 20:59, 26 September 2012
Contents |
NEB buffers
Composition of NEBuffers (1x) as provided by their website [1]:
NEBuffer 1 (yellow)
- 10 mM Bis Tris Propane-HCl
- 10 mM MgCl2
- 1 mM DTT (pH 7.0 at 25°C).
NEBuffer 2 (blue)
- 10 mM Tris-HCl
- 10 mM MgCl2
- 50 mM NaCl
- 1 mM DTT (pH 7.9 at 25°C)
NEBuffer 3 (red)
- 50 mM Tris-HCl
- 10 mM MgCl2
- 100 mM NaCl
- 1 mM DTT (pH 7.9 at 25°C)
NEBuffer 4 (green)
- 20 mM Tris-acetate
- 10 mM magnesium acetate
- 50 mM potassium acetate
- 1 mM DTT (pH 7.9 at 25°C)
T4 DNA Ligase Buffer
- 50 mM Tris-HCl
- 10 mM MgCl2
- 1 mM ATP
- 10 mM Dithiothreitol (pH 7.5 at 25°C)
NEBuffer EcoRI
- 100 mM Tris-HCl
- 50 mM NaCl
- 10 mM MgCl2
- 0.025 % Triton X-100 (pH 7.5 at 25°C)
References
- NEB http://www.neb.com/nebecomm/tech_reference/restriction_enzymes/buffer_legend.asp
