Team:TU Darmstadt/Materials/Materials

From 2012.igem.org

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(Sigma-Aldrich Co. LLC.)
(CARL ROTH GMBH + CO. KG)
 
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Line 39: Line 39:
=== CARL ROTH GMBH + CO. KG ===
=== CARL ROTH GMBH + CO. KG ===
* acetic acid
* acetic acid
 +
* acetone
* acrylamide (rotiphorese)
* acrylamide (rotiphorese)
* agar-agar kobe I
* agar-agar kobe I
Line 50: Line 51:
* ethanol
* ethanol
* ethylenediaminetetraacetic acid (EDTA)
* ethylenediaminetetraacetic acid (EDTA)
 +
* ethylacetate
* gloves
* gloves
* glycerol
* glycerol
* HCl
* HCl
 +
* hexane
* imidazole
* imidazole
* isopropyl alcohol
* isopropyl alcohol
Line 59: Line 62:
* KH<sub>2</sub>PO<sub>4</sub>
* KH<sub>2</sub>PO<sub>4</sub>
* L-(+)-arabinose
* L-(+)-arabinose
 +
* methanol
* Na<sub>2</sub>HPO<sub>4</sub>
* Na<sub>2</sub>HPO<sub>4</sub>
* NaCl
* NaCl
Line 64: Line 68:
* NiCl<sub>2</sub>
* NiCl<sub>2</sub>
* sodiumdodecylsulfate (SDS)
* sodiumdodecylsulfate (SDS)
 +
* sulfuric acid 96% (w/v)
* tributyrine
* tributyrine
* tricine
* tricine
 +
* triethylamine
* TRIS-HCl
* TRIS-HCl
* tryptone
* tryptone
* yeast extract
* yeast extract
 +
=== AppliChem GmbH  ===
=== AppliChem GmbH  ===
* bromothymol blue
* bromothymol blue
Line 75: Line 82:
=== Sigma-Aldrich Co. LLC. ===
=== Sigma-Aldrich Co. LLC. ===
* bromphenol blue
* bromphenol blue
 +
* dimethylterephtalate
* hydrocinnamoyl chloride
* hydrocinnamoyl chloride
* para-nitrophenol
* para-nitrophenol

Latest revision as of 21:10, 26 September 2012

Contents

Chemicals

CARL ROTH GMBH + CO. KG

  • acetic acid
  • acetone
  • acrylamide (rotiphorese)
  • agar-agar kobe I
  • ammonium persulfate (APS)
  • ammonium sulfate
  • ampicillin
  • ß-mercaptoethanol
  • chloramphenicol
  • coomassie brillant blue 250
  • D-(+)-glucose
  • ethanol
  • ethylenediaminetetraacetic acid (EDTA)
  • ethylacetate
  • gloves
  • glycerol
  • HCl
  • hexane
  • imidazole
  • isopropyl alcohol
  • kanmycin
  • KCl
  • KH2PO4
  • L-(+)-arabinose
  • methanol
  • Na2HPO4
  • NaCl
  • NaOH
  • NiCl2
  • sodiumdodecylsulfate (SDS)
  • sulfuric acid 96% (w/v)
  • tributyrine
  • tricine
  • triethylamine
  • TRIS-HCl
  • tryptone
  • yeast extract

AppliChem GmbH

  • bromothymol blue
  • tetramethylethylenediamine (TEMED)

Sigma-Aldrich Co. LLC.

  • bromphenol blue
  • dimethylterephtalate
  • hydrocinnamoyl chloride
  • para-nitrophenol
  • succinyl chloride
  • tween 20

New England Biolabs GmbH

Enzymes

  • EcoRI
  • EcoRI HF
  • NcoI HF
  • NdeI HF
  • NotI HF
  • Phusion
  • PstI HF
  • Q5 Polymerase Hotstart
  • Q5 Polymerase normal
  • T4 DNA Ligase
  • XbaI HF

Buffer

  • 2-log DNA marker
  • bovine serum albumin (BSA 100x)
  • Buffer 4
  • dimethylsulfoxide (DMSO)
  • DNA loading Dye 6x
  • GC Phusion 5x Buffer
  • GC Q5 Enhancer Buffer
  • MgCl2
  • Phusion HF Buffer 5x
  • Q5 reaction Buffer
  • T4 Ligase Buffer 10x

Promega GmbH

Enzymes

  • EcoRI
  • PstI
  • SpeI GQ
  • T4 Ligase
  • TEV Protease
  • XbaI GQ

Buffer

  • Buffer B
  • Buffer D
  • Buffer H
  • Go Tag Puffer 5x
  • Go Tag Puffer 5x prestained
  • Multi Core 10x Buffer
  • T4 Ligase Buffer 10x

Kits

  • Pure Yield Plasmid Miniprep System
  • Wizard SV Gel and PCR Clean Up System