Team:TMU-Tokyo/Notebook/Assay

From 2012.igem.org

Revision as of 02:15, 27 September 2012 by Hiroyuki (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

 




■Experiment
1st week (8.13 - 8.19)
2nd week (8.20 - 8.26)
3rd week (8.27 - 9. 2)
4th week (9. 3 - 9. 9)
5th week (9.10 - 9.16)
6th week (9.17 - 9.23)
7th week (9.24 - 9.30)


■Protocols


■Assay
Assay0
Device1 Assay
Device2 Assay
Device3 Assay




Assay



■Assay0
Resistant formaldehyde and formate
Assay 0 Protocol and Result


■Assay1(Device1)
Detect formaldehyde
Assay 1 Protocol and Result


■Assay2(Device2)
Resistant formaldehyde and Quantitative Analysis of Formaldehyde by Formaldehyde Test wako
Assay 2 Protocol and Result


■Assay3(Device3)
・Quantitative Analysis of formate by high performance liquid chromatography(HPLC)
・FDH4 requires NAD⁺ when formate dehydrogenate. So FDH activitiy Ant E. coli produces can be measured by reduction of NAD⁺ at 340nm.
Assay 3 Protocol and Result