Team:TMU-Tokyo

From 2012.igem.org

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   <p class="title">Project Abstract</H1></p>
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   <p class="title">Wiki map</H1></p>
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Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials)<Br>
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<b>■<A Href="https://2012.igem.org/Team:TMU-Tokyo/Team">About us</A></b><Br>
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Also, since formaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. This year, in Japan, the detection of formaldehyde in Tone river became an issue.<Br>
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Introduction of our team, university, member.<Br>
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We planed to create <i>E. coli</i> with an ability to　detect and detoxify formaldehyde. named <b>Chef Ant E. coli</b>.
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<b>■<A Href="https://2012.igem.org/Team:TMU-Tokyo/Project">Project</A></b><Br>
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About detection, we try to visualize formaldehyde by ligating regulated promoter, <i>frmR</i> and GFP.
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Details of our project <b>Chef Ant E. coli</b>.<Br>
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Moreover we plan to overexpress two enzymes in <b>Chef Ant E. coli</b>.<Br>
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First, formaldehyde dehydrogenase decomposes formaldehyde to formate. The gene of formaldehyde dehydrogenase is from <i>Pseudomonas Putida</i>.<Br>
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Second, formate dehydrogenase converts formate to CO₂ and H₂O. The gene of formate dehydrogenase is from <i>Methylbacterium extorquens</i>. <Br>
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More information is available <A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_abstract">here</A>.
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<b>■<A Href="https://2012.igem.org/Team:TMU-Tokyo/Parts">Parts</A></b><Br>
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Details of parts we made.<Br>
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<b>■<A Href="https://2012.igem.org/Team:TMU-Tokyo/Notebook">Notebook</A></b><Br>
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Experiment and protocols, assay we did.<Br>
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<b>■<A Href="https://2012.igem.org/Team:TMU-Tokyo/Safety>Human&Safety</A></b><Br>
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Human and Safety activity we did.<Br>
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Revision as of 09:34, 23 September 2012



Welcome to Team; TMU-Tokyo wiki!

Our project is Chef Ant E. coli, Escherichia coli transformeted formaldehyde detoxification function.
The first of formaldehyde detoxification pathway is conversion formaldehyde to formate, as the right image. Chef Ant E. coli was named after this stage, formate was first purified from ants and farther formate conversion is seemed like ant eater.






Wiki map




■About us
Introduction of our team, university, member.

■Project
Details of our project Chef Ant E. coli.

■Parts
Details of parts we made.

■Notebook
Experiment and protocols, assay we did.

■



Image of Chef Ant E.coli.


Retrieved from "http://2012.igem.org/Team:TMU-Tokyo"