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Team:TMU-Tokyo
From 2012.igem.org
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| - | Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials) Also, since formaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. This year, in Japan, the detection of formaldehyde in Tone river became an issue.<Br> | + | Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials)<Br> |
| + | Also, since formaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. This year, in Japan, the detection of formaldehyde in Tone river became an issue.<Br> | ||
<Br> | <Br> | ||
| - | We planed to create E.coli with an ability to detect and detoxify formaldehyde. named Chef Ant E.coli. | + | We planed to create <i>E. coli</i> with an ability to detect and detoxify formaldehyde. named <b>Chef Ant E. coli</b>. |
| - | About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP. | + | About detection, we try to visualize formaldehyde by ligating regulated promoter, <i>frmR</i> and GFP. |
| - | Moreover we plan to overexpress two enzymes in Chef Ant E.coli.<Br> | + | Moreover we plan to overexpress two enzymes in <b>Chef Ant E. coli</b>.<Br> |
| - | First, formaldehyde dehydrogenase decomposes formaldehyde to | + | First, formaldehyde dehydrogenase decomposes formaldehyde to formate. The gene of formaldehyde dehydrogenase is from <i>Pseudomonas Putida</i>.<Br> |
| - | Second, | + | Second, formate dehydrogenase converts formate to CO₂ and H₂O. The gene of formate dehydrogenase is from <i>Methylbacterium extorquens</i>. <Br> |
| + | <Br> | ||
| + | More information is available <A Href="https://2012.igem.org/Team:TMU-Tokyo/Project_abstract">here</A>. | ||
</p> | </p> | ||
Revision as of 18:16, 21 September 2012
Our team project Chef Ant E.coli
Project Abstract
Formaldehyde is a common harmful chemical, and it has a bad effect in relatively low concentration. (For example, in agricultural chemicals, in disinfectant at hospitals and in paint of building materials)
Also, since formaldehyde is mass-produced in factories, it is highly possible to exceed over the permissible amount in the environment. This year, in Japan, the detection of formaldehyde in Tone river became an issue.
We planed to create E. coli with an ability to detect and detoxify formaldehyde. named Chef Ant E. coli.
About detection, we try to visualize formaldehyde by ligating regulated promoter, frmR and GFP.
Moreover we plan to overexpress two enzymes in Chef Ant E. coli.
First, formaldehyde dehydrogenase decomposes formaldehyde to formate. The gene of formaldehyde dehydrogenase is from Pseudomonas Putida.
Second, formate dehydrogenase converts formate to CO₂ and H₂O. The gene of formate dehydrogenase is from Methylbacterium extorquens.
More information is available here.
File:TMU-Tokyo logo.png 200px | |
File:TMU-Tokyo team.png Your team picture | |
| Team TMU-Tokyo |
