Team:Slovenia/ModelingInteractiveSimulations

From 2012.igem.org

(Difference between revisions)
Line 437: Line 437:
       <tr class="normal"><td class="normal"><strong>[B]_pCMV_TAL-A:KRAB_mCitrine</strong> </td><td class="normal"> <input type="text" name="plasmid2" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
       <tr class="normal"><td class="normal"><strong>[B]_pCMV_TAL-A:KRAB_mCitrine</strong> </td><td class="normal"> <input type="text" name="plasmid2" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
       <tr class="normal"><td class="normal"><strong>pCMV_[PIR]_TAL-B:KRAB</strong> </td><td class="normal"> <input type="text" name="plasmid3" size="5" value="190" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
       <tr class="normal"><td class="normal"><strong>pCMV_[PIR]_TAL-B:KRAB</strong> </td><td class="normal"> <input type="text" name="plasmid3" size="5" value="190" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
-
       <tr class="normal"><td class="normal"><strong>pCMV_[ETR]_TAL-B:KRAB</strong> </td><td class="normal"> <input type="text" name="plasmid4" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
+
       <tr class="normal"><td class="normal"><strong>pCMV_[ETR]_TAL-A:KRAB</strong> </td><td class="normal"> <input type="text" name="plasmid4" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
       <tr class="normal"><td class="normal"><strong>pCMV_PIP:KRAB</strong> </td><td class="normal"> <input type="text" name="plasmid5" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
       <tr class="normal"><td class="normal"><strong>pCMV_PIP:KRAB</strong> </td><td class="normal"> <input type="text" name="plasmid5" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
       <tr class="normal"><td class="normal"><strong>pCMV_E:KRAB </strong> </td><td class="normal"> <input type="text" name="plasmid6" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>
       <tr class="normal"><td class="normal"><strong>pCMV_E:KRAB </strong> </td><td class="normal"> <input type="text" name="plasmid6" size="5" value="180" onKeyPress="return numbersonly(this, event)" maxlength="4"/> ng</td></tr>

Revision as of 17:02, 25 September 2012


Quantitative model interactive simulations


Based on experimental data, we constructed what we refer to as a quantitative model. Please see Quantitative and stability model for details.



Mutual repressor switch

INPUT VECTORS DOSAGE
ConstructDosage
[A]_pCMV_TAL-B:KRAB_BFP ng
[B]_pCMV_TAL-A:KRAB_mCitrine ng
pCMV_[PIR]_TAL-B:KRAB ng
pCMV_[ETR]_TAL-A:KRAB ng
pCMV_PIP:KRAB ng
pCMV_E:KRAB ng
(Enter dosages for corresponding plasmids.)


TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)


INDUCERS
Pristinamycin
Input times [min]
Durations [min]





Erithromycin
Input times [min]
Durations [min]
(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )

SIMULATION

Time: min
(Simulation running time)


(The results will be shown in a new browser window/tab after few seconds. Have fun.)


Positive feedback loop switch

INPUT VECTORS DOSAGE
ConstructDosage
[A]_pCMV_TAL-B:KRAB_TAL-A:VP16_BFP ng
[B]_pCMV_TAL-A:KRAB_TAL-B:VP16_mCitrine ng
pCMV_[PIR]_TAL-B:KRAB_TAL-A:VP16 ng
pCMV_[ETR]_TAL-A:KRAB_TAL-B:VP16 ng
pCMV_PIP:KRAB_E:KRAB ng
(Enter dosages for corresponding plasmids.)


TRANSCRIPTION FACTORS
Equal transcription factors
(Transcription factors will alter transcription with equal rates)


INDUCERS
Pristinamycin
Input times [min]
Durations [min]





Erithromycin
Input times [min]
Durations [min]
(For each input time of an inducer, enter its corresponding duration, i.e. after how many minutes the inducer will be removed. )


SIMULATION
Time: min
(Simulation running time)


(The results will be shown in a new browser window/tab after few seconds. Have fun.)