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Team:Shenzhen/Temp/media project yao suicider.htm
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(Created page with "<!---------------------- Title: media_project_yao_suicider.htm Team: Shenzhen Author: BGI_K2 Twitter: @BGI_K2 Email: im.ss.kk@gmail.com Please contact me if you would like...") |
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<li><img data-frame="https://static.igem.org/mediawiki/2012/7/74/Yao.028-001.jpg" src="https://static.igem.org/mediawiki/2012/7/74/Yao.028-001.jpg" title="YAO.Suicider" data-description="Project description to YAO.Suicider." /> | <li><img data-frame="https://static.igem.org/mediawiki/2012/7/74/Yao.028-001.jpg" src="https://static.igem.org/mediawiki/2012/7/74/Yao.028-001.jpg" title="YAO.Suicider" data-description="Project description to YAO.Suicider." /> | ||
| - | <li><img data-frame="https:// | + | <li><img data-frame="https://static.igem.org/mediawiki/2012/d/d9/Project_yao_suicider_p3.jpg" src="https://static.igem.org/mediawiki/2012/d/d9/Project_yao_suicider_p3.jpg" title="T7 Imitation System" data-description="Verify the function of T7 RNAP/Promoter system in cytoplasm. If it works, fluorescence of GFP can be detected in cytoplasm.." /> |
<li><img data-frame="https://static.igem.org/mediawiki/2012/c/c3/Project_yao_suicider_p4.jpg" src="https://static.igem.org/mediawiki/2012/c/c3/Project_yao_suicider_p4.jpg" title="T7 Imitation System" data-description="Verify the function of T7 RNAP/Promoter system in mitochondrion or unfinished YAO, basing on the availability of signal peptide from cytoplasm to mitochondrial matrix. If it works, fluorescence of mtGFP can be detected in mitochondrion." /> | <li><img data-frame="https://static.igem.org/mediawiki/2012/c/c3/Project_yao_suicider_p4.jpg" src="https://static.igem.org/mediawiki/2012/c/c3/Project_yao_suicider_p4.jpg" title="T7 Imitation System" data-description="Verify the function of T7 RNAP/Promoter system in mitochondrion or unfinished YAO, basing on the availability of signal peptide from cytoplasm to mitochondrial matrix. If it works, fluorescence of mtGFP can be detected in mitochondrion." /> | ||
<li><img data-frame="https://static.igem.org/mediawiki/2012/f/fb/Project_yao_suicider_p5.jpg" src="https://static.igem.org/mediawiki/2012/f/fb/Project_yao_suicider_p5.jpg" title="Retrograde Signaling" data-description="Verify the basal expression of gene downstream DLD3 promoter. Roughly judge the brightness of GFP, and consequently select a suitable degradation tag for gene downstream DLD3 promotor." /> | <li><img data-frame="https://static.igem.org/mediawiki/2012/f/fb/Project_yao_suicider_p5.jpg" src="https://static.igem.org/mediawiki/2012/f/fb/Project_yao_suicider_p5.jpg" title="Retrograde Signaling" data-description="Verify the basal expression of gene downstream DLD3 promoter. Roughly judge the brightness of GFP, and consequently select a suitable degradation tag for gene downstream DLD3 promotor." /> | ||
<li><img data-frame="https://2012.igem.org/File:Project_yao_suicider_p6.jpg" src="https://2012.igem.org/File:Project_yao_suicider_p6.jpg" title="Retrograde Signaling" data-description="Verify the function of selected degradation tag by using UV as a imitation of DNase produced in mitochondrion that can lead to mitochondrion dysfunction which will drive the gene expression downstream DLD3 promoter through Retrograde signaling from mitochondrion to nucleus." /> | <li><img data-frame="https://2012.igem.org/File:Project_yao_suicider_p6.jpg" src="https://2012.igem.org/File:Project_yao_suicider_p6.jpg" title="Retrograde Signaling" data-description="Verify the function of selected degradation tag by using UV as a imitation of DNase produced in mitochondrion that can lead to mitochondrion dysfunction which will drive the gene expression downstream DLD3 promoter through Retrograde signaling from mitochondrion to nucleus." /> | ||
| - | <li><img data-frame="https:// | + | <li><img data-frame="https://static.igem.org/mediawiki/2012/f/f8/Project_yao_suicider_p7.jpg" src="https://static.igem.org/mediawiki/2012/f/f8/Project_yao_suicider_p7.jpg" title="Retrograde Signaling" data-description="Verify that holing can make pores on the inner membrane of mitochondrion. This is the alterant of DNase causing mt dysfunction. And it can also produce a membrane without mtDNA." /> |
| - | <li><img data-frame="https:// | + | <li><img data-frame="https://static.igem.org/mediawiki/2012/1/19/Project_yao_suicider_p8.jpg" src="https://static.igem.org/mediawiki/2012/1/19/Project_yao_suicider_p8.jpg" title="For Host Cell Suicide Part" data-description="Verify the function of holing in yeast to prove that holing can act as bax/bak protein in human cell as a apoptosis activator. If it works, we can see that between the engineered and versus one, the growthrate curve (OD600) of engineered one tend to be lower than that of versus one." /> |
</ul> | </ul> | ||
</body> | </body> | ||
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Revision as of 04:03, 27 September 2012
