Team:Shenzhen/Notebook/YAO.Genome

Journal

• Apr 17th, 2012

YAO. Genome Seminar: Papers from Yu CHEN should be read CAREFULLY which are mainly about plasmid introduction to mitochondrial.

Problems to be solved:

1. Experiment principle;

2. Why necessary to each step;

3. Bacterial strain to be used;

4. Culture to be used.

Core Papers:

1. Fox et al. SCIENCE, VOL. 240. Mitochondrial Transformation in Yeast by Bombardment with Microprojectiles.

2. STEPHEN A. JOHNSTON et al. Proc. Natl. Acad. Sci. USA 85 (1988). Plasmids can stably transform yeast mitochondria lacking endogenous mtDNA

Participants:

Junguang XU, Qiwu XU, Haojun JIANG, Jie WANG, Qiong WU, Senwei TANG, Qinsi LIANG, Min OU.

• Apr 20th, 2012

YAO. Genome experiment design for first part:

Task assignment:

1. Usage of Gene gun and fluorescence microscope

2. Research on mitochondria transformation methods

3. Research on Saccharomyces cerevisiae genome, especially transcription system.

4. Research on plasmid transformation directly or after isolated mitochondria.

5. Research on detection methods about whether transformation is succeed.

Core paper:

Nathalie Bonnefoy et al. METHODS IN CELL BIOLOGY, VOL. 80. Genetic Transformation of Saccharomyces cerevisiae and Chlamydomonas reinhardtii Mitochondria.

Participants:

Junguang XU, Qiwu XU, Haojun JIANG, Jie WANG, Qiong WU, Senwei TANG, Qinsi LIANG, Min OU.

• Apr 21st, 2012

YAO. Genome experiment design first draft came out.

Participants: Qiwu XU

• May 5th, 2012

Primers and vectors designation.

Participants: Qiwu XU, Min YANG, Yun WANG

• May 24th, 2012

Junguang XU shared a correlated paper:

de Jong et al. BMC Systems Biology 2010, 4:55 Methodology article Experimental and computational validation of models of fluorescent and luminescent reporter genes in bacteria.

• May 27th, 2012

Start to synthesize primers, vectors and other parts, such as promoters and terminators.

Participants: Qiwu XU, Min YANG, Yun WANG

• Jun 8th, 2012

Culture media compilation

Preparations for pour plate method and vectors transformation.

Participants: Qiwu XU, Senwei TANG

• Jun 9th, 2012

Transformation experiment

Participants: Qiwu XU, Senwei TANG

• Jun 10th, 2012

PCR and electrophoresis

Bacterial growth

Participants: Qiwu XU, Senwei TANG

• Jun 11th, 2012

Sequencing

Participants: Qiwu XU, Senwei TANG

• Jun 12th, 2012

Assignments for this weeks experiments

Participants: Qiwu XU

• Jun 13th, 2012

Culture media compilation

Night 20:00 - 22:00

Yeast growth

Participants: Qiwu XU, Senwei TANG

• Jun 14th, 2012 Night

Pour plate method processed.

Participants: Qiwu XU, Senwei TANG

• Jun 15th, 2012 Night

Reagents preparation

Participants: Qiwu XU, Senwei TANG

• Jun 16th - 17th, 2012

Preparations for latter experiments

Participants: Qiwu XU, Senwei TANG

• Jul 2rd - Sep 1st, 2012

YAO. Genome story, pictures preparation and presentation design.

Participants:

Junguang XU, Qiwu XU, Haojun JIANG, Jie WANG, Qiong WU, Senwei TANG, Qinsi LIANG, Junjie Zhang, Min OU.

• Sep 6th, 2012

Scripts, BioBricks and pictures preparation for Wiki

Participants:

Qiwu XU, Min OU.