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Team:Shenzhen/Biobricks
From 2012.igem.org
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BioBricks
- Summary
- YAO.Genome
- YAO.Channel
- YAO.Sensor
- YAO.Suicider
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Notebook
- Team History
- YAO.Genome
- YAO.Channel
- YAO.Sensor
- YAO.Suicider
- YAO.Factory
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Practices
- Best Parts
- Best Parts, Engineered
- Best Improved Parts
Favorite Parts
BBa_K809001, mitochondrial coded GFP
BBa_K809002, mitochondrial replication origin ori7
BBa_K809003, mitochondrial upstream elements (promoter+RBS), from cox1 gene
BBa_K809101, mtGFP expression unit
BBa_K809605, lamda-Holin in BBa format, engineered from BBa_K112306 in BBb format
BBa_K809601, GAL promoter without start codon, engineered from BBa_J63006
Table 1. Favorite parts in project YAO #1.0.
| -?- | Name | Type | Description | Designer | Length | ||
|---|---|---|---|---|---|---|---|
| W | BBa_K809001 | Reporter | Mitochondrial GFP | Qiwu XU | 717 | ||
| W | BBa_K809002 | Other | ori7 region of mtDNA | Qiwu XU | 378 | ||
| W | BBa_K809003 | Regulatory | Up elements of cox1 gene | Qiwu XU | 497 | ||
| W | BBa_K809101 | Composite | Mitochondrial shuttle plasmid carried mtGFP | Senwei TANG, Qiwu XU | 1606 | ||
| W | BBa_K809601 | Regulatory | GAL promoter (kozak) without start codon ATG | Jianhui Gong | 549 | ||
| W | BBa_K809605 | Coding | Lambda Holin in BBa format | Jianhui Gong | 318 |
All Parts
We have designed and constructed dozens of BioBricks for the five sub-projects. Some of them have been submitted to PartsRegistry, and some of them have been proved workable.
Table 2. All designed parts in project YAO #1.0.
| -?- | Name | Type | Description | Designer | Length | ||
|---|---|---|---|---|---|---|---|
| BBa_K809004 | Terminator | Terminator of Yeast mitochondrial gene COB | Xiaopeng Xu | 50 | |||
| BBa_K809005 | Terminator | Terminator of Yeast mitochondrial gene COX3 | Xiaopeng Xu | 49 | |||
| BBa_K809006 | Terminator | Terminator of Yeast mitochondrial gene Q0255 | Xiaopeng Xu | 65 | |||
| BBa_K809007 | Regulatory | Promoter of Yeast mitochondrial gene COX1/Oli1/15s rRNA | Xiaopeng Xu | 11 | |||
| BBa_K809008 | RBS | RBS sequence of yeast mitochondrial gene AI1 | Xiaopeng Xu | 54 | |||
| BBa_K809009 | RBS | RBS sequence of yeast mitochondrial gene OLI1 | Xiaopeng Xu | 114 | |||
| W | BBa_K809010 | DNA | Upstream recombination site of cox3 | Kang KANG | |||
| W | BBa_K809011 | DNA | Dowstream recombination site of cox3 | Kang KANG | |||
| W | BBa_K809102 | Composite | Recombination sequence carried with mtGFP | Kang KANG | |||
| BBa_K809103 | Composite | Original termination efficiency test BioBricks for Tcob | Kang KANG | 1278 | |||
| BBa_K809104 | Composite | Original termination efficiency test BioBricks for Tcox3 | Kang KANG | 1277 | |||
| BBa_K809105 | Composite | Original termination efficiency test BioBricks for Tq0255 | Kang KANG | 1293 | |||
| BBa_K809106 | Coding | Efficiency test of COX3 Terminator | Xiaopeng Xu | 911 | |||
| BBa_K809107 | Coding | Efficiency test of COB Terminator | Xiaopeng Xu | 912 | |||
| BBa_K809108 | Coding | Efficiency test of Q0255 Terminator | Xiaopeng Xu | 911 | |||
| BBa_K809201 | Protein_Domain | signal peptide of ZIM17 | Luohao Xu | 141 | |||
| BBa_K809202 | Protein_Domain | signal peptide of FER | Luohao Xu | 96 | |||
| BBa_K809203 | Protein_Domain | signal peptide of TIM21 | Luohao Xu | 96 | |||
| BBa_K809204 | Protein_Domain | signal peptide of Tom40 | Luohao Xu | 147 | |||
| BBa_K809205 | Protein_Domain | signal peptide of Tom70 | Luohao Xu | 90 | |||
| BBa_K809206 | Protein_Domain | signal peptide of Tom22 | Luohao Xu | 130 | |||
| BBa_K809207 | Protein_Domain | signal peptide of Tom20 | Luohao Xu | 120 | |||
| BBa_K809208 | Coding | Toc34 | Luohao Xu | 1467 | |||
| BBa_K809209 | Coding | Toc75 | Luohao Xu | 2520 | |||
| BBa_K809301 | Reporter | RFP + signal peptide of ZIM17 | Luohao Xu | 853 | |||
| BBa_K809302 | Reporter | RFP + signal peptide of FER | Luohao Xu | 808 | |||
| BBa_K809303 | Reporter | RFP + signal peptide of TIM21 | Luohao Xu | 808 | |||
| BBa_K809304 | Reporter | RFP + signal peptide of Tom40 | Luohao Xu | 859 | |||
| BBa_K809305 | Reporter | RFP + signal peptide of Tom70 | Luohao Xu | 802 | |||
| BBa_K809306 | Reporter | RFP + signal peptide of Tom22 | Luohao Xu | 842 | |||
| BBa_K809308 | Coding | GAL promoter + RFP_SPzim17 + ADH1 terminator | Luohao Xu | 1642 | |||
| BBa_K809309 | Coding | GAL promoter + RFP_SPfer + ADH1 terminator | Luohao Xu | 1597 | |||
| BBa_K809310 | Coding | GAL promoter + RFP_SPtim21 + ADH1 terminator | Luohao Xu | 1642 | |||
| BBa_K809311 | Coding | GAL promoter + RFP_SPtom70 + ADH1 terminator | Luohao Xu | 1591 | |||
| BBa_K809312 | Coding | GAL promoter + RFP_SPtom22 + ADH1 terminator | Luohao Xu | 1633 | |||
| BBa_K809313 | Reporter | YFP + signal peptide of ZIM17 | Luohao Xu | 179 | |||
| BBa_K809314 | Reporter | YFP + signal peptide of FER | Luohao Xu | 134 | |||
| BBa_K809315 | Reporter | YFP + signal peptide of Tim21 | Luohao Xu | 134 | |||
| BBa_K809316 | Reporter | YFP + signal peptide of Tom40 | Luohao Xu | 185 | |||
| BBa_K809317 | Reporter | YFP + signal peptide of Tom70 | Luohao Xu | 128 | |||
| BBa_K809318 | Reporter | YFP + signal peptide of Tom22 | Luohao Xu | 158 | |||
| BBa_K809319 | Coding | GAL promoter + YFP_SPzim17 + ADH1 terminator | Luohao Xu | 967 | |||
| BBa_K809320 | Coding | GAL promoter + YFP_SPfer + ADH1 terminator | Luohao Xu | 922 | |||
| BBa_K809321 | Coding | GAL promoter + YFP_SPtim21 + ADH1 terminator | Luohao Xu | 922 | |||
| BBa_K809322 | Coding | GAL promoter + YFP_SPtom70 + ADH1 terminator | Luohao Xu | 916 | |||
| BBa_K809323 | Coding | GAL promoter + YFP_SPtom22 + ADH1 terminator | Luohao Xu | 946 | |||
| BBa_K809324 | Coding | signal peptide of Tom70 + Toc34 | Luohao Xu | 1563 | |||
| BBa_K809325 | Coding | YFP + signal peptide of Tom70 + Toc34 | Luohao Xu | 1599 | |||
| BBa_K809326 | Coding | signal peptide of Tom40 + Toc75 | Luohao Xu | 2673 | |||
| BBa_K809327 | Coding | YFP + signal peptide of Tom40 + Toc75 | Luohao Xu | 2711 | |||
| BBa_K809328 | Coding | GAL promoter + Toc34_SPtom70 + ADH1 terminator | Luohao Xu | 2351 | |||
| BBa_K809329 | Coding | GAL promoter + Toc75_SPtom40 + ADH1 terminator | Luohao Xu | 3463 | |||
| BBa_K809401 | Coding | REX coding sequence | Xiaopeng Xu | 777 | |||
| BBa_K809402 | Regulatory | Promoter+ROP in mitochondria | Xiaopeng Xu | 43 | |||
| BBa_K809403 | Regulatory | ROP+Promoter in mitochondria | Xiaopeng Xu | 43 | |||
| BBa_K809406 | Coding | P+ROP+OLI1 RBS+mtGFP+COX3 terminator | Xiaopeng Xu | 943 | |||
| BBa_K809501 | Coding | REX+SPzim17 | Xiaopeng Xu | 924 | |||
| BBa_K809502 | Coding | GAL promoter + SP_REX + ADH1 terminator | Xiaopeng Xu | 1713 | |||
| BBa_K809504 | Coding | ROP+P+OLI1 RBS+mtGFP+COX3 terminator | Xiaopeng Xu | 943 | |||
| BBa_K809602 | Coding | T7 RNAP | Jianhui Gong | 2652 | |||
| BBa_K809603 | Coding | Bovine Pancreatic DNase I (in yeast mt codon table) | Jianhui Gong | 3023 | |||
| BBa_K809604 | Coding | Bovine Pancreatic DNase I | Jianhui Gong | 3023 | |||
| BBa_K809606 | Regulatory | DLD3 promoter | Jianhui Gong | 1939 | |||
| BBa_K809607 | Coding | human bax protein | Jianhui Gong | 6942 | |||
| BBa_K809701 | Coding | SP of ZIM17 + T7 RNAP | Jianhui Gong | 2799 | |||
| BBa_K809702 | Coding | SP of ZIM17 + Bovine Pancreatic DNase I | Jianhui Gong | 3172 | |||
| BBa_K809703 | Coding | SP of ZIM17 + Holin (lambda phage) | Jianhui Gong | 465 | |||
| BBa_K809704 | Coding | Lambda Holin + hsl1 degradation tag | Jianhui Gong | 944 | |||
| BBa_K809711 | Coding | GAL promoter + kozak + ZIM17+ T7 RNAP + ADH1 terminator | Jianhui Gong | 3587 | |||
| BBa_K809712 | Coding | GAL promoter + kozak + T7 RNAP + ADH1 terminator | Jianhui Gong | 3440 | |||
| BBa_K809713 | Coding | T7 promoter + yeast mt RBS(AI1)+ GFP + ADH1 terminator | Jianhui Gong | 1042 | |||
| BBa_K809714 | Coding | T7 promoter + yeast mt RBS(AI1) + mtGFP + ADH1 terminator | Jianhui Gong | 1039 | |||
| BBa_K809715 | Coding | T7 promoter + yeast mt RBS(AI1)+ Bovine Pancreatic DNase I (mt) + ADH1 terminator | Jianhui Gong | 3347 | |||
| BBa_K809716 | Coding | GAL promoter + DNase + ADH1 terminator | Jianhui Gong | 3813 | |||
| BBa_K809717 | Coding | GAL promoter + ZIM17 + DNase(mt) + ADH1 terminator | Jianhui Gong | 3960 | |||
| BBa_K809718 | Coding | GAL promoter + ZIM17 + hoiln(lambda phage) + ADH1 terminator | Jianhui Gong | 1253 | |||
| BBa_K809719 | Coding | DLD3 promoter + holin(lambda phage) + hsl1 tag + ADH1 terminator | Jianhui Gong | 3148 | |||
| BBa_K809720 | Coding | DLD3 promoter + kozak + yeast GFP+ ADH1 terminator | Jianhui Gong | 2924 | |||
| BBa_K809721 | Coding | DLD3 promoter + kozak + hoiln(lambda phage) + ADH1 terminator | Jianhui Gong | 2522 | |||
| BBa_K809722 | Coding | DLD3 promoter + kozak + yeast GFP + hsl1 tag +ADH1 terminator | Jianhui Gong | 2229 | |||
| BBa_K809723 | Coding | GAL promoter + kozak + hoiln(lambda phage)+ ADH1 terminator | Jianhui Gong | 2522 | |||
| BBa_K809724 | Coding | GAL promoter + kozak + Bax(human) + ADH1 terminator | Jianhui Gong | 9148 |
