Team:SUSTC-Shenzhen-B/week 3

From 2012.igem.org

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             <div class="col col23">
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                 <h2>July</h2>
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                 <h2>September</h2>
                 <h3>Abstract</h3>
                 <h3>Abstract</h3>
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                 <p>This week we plan to have a short vacation and discuss the next step of our project.</p>
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                 <p>From this week we paid more attention to our human practices</p>
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                <h3>Week 3</h3>
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                <h3>7.29.2012-7.31.2012</h3>
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<p>7.29.2012</p>
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<p>We took a break to get ready for the week ahead of us.</p>
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<br>
<br>
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<p>7.30.2012</p>
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                <h3>Week 2</h3>
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<p>We moved our work place from school to BGI to do some experiment there. All of us were excited about the move.</p>
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                <h3>9.16.2012-9.22.2012</h3>
 +
<p>9.17.2012</p>
 +
<p>After all the material we bought arrived, we started our lab work again in our school. It’s a hard work to develop our own laboratory. But we had fun at the same time.</p>
 +
<p>We did a digest and amplify GFP & RFP with PCR.
 +
</p>
<br>
<br>
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<p>7.31.2012</p>
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<p>9.18.2012-9.20.2012</p>
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<p>1. We divided our team members into 2 groups. One will be focusing on reading paper and looking for the right terminator; the other one will be focusing on designing and doing experiment.</p>
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<p>We visited 3 high schools and gave presentations to their students on synthetic biology and our IGEM projects. There are many students being interested in biology and some of them would like to join this competition. We think our major goal has been achieved.
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<p>2. Our adviser Chen Yu gave us an instruction session an molecular biology to help us get started our work.</p>
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We also did a ligation to connect GFP, RFP and the plasmid together. Then we did a transformation.
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<p>3. Moving to a new place is not an easy thing. We cleaned our dormitory. </p>
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</p>
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<br>
 +
<p>9.21.2012</p>
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<p>Today’s morning, we saw that control group almost exist only few of bacterial colony and the experimental group grow lots of colonies. I think it is a positive suggest. So we did a colony PCR to test whether the ligation is successful. We picked up 10 colonies to test and three of them is positive ligated by three parts and all the colonies are positive ligated by two parts.</p>
 +
<br>
 +
<p>9.22.2012</p>
 +
<p>We picked up those colonies which are positive.  Through the bacterium culture in shake-flask more than 12 hours, we can do plasmid miniprep.</p>
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<br>
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                 <h2>Week Diary</h2>
                 <h2>Week Diary</h2>
                 <ul class="tmo_ul_list">
                 <ul class="tmo_ul_list">
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                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/july">week 1</a></li>
+
                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/"september>week 1</a></li>
                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/week 2">week 2</a></li>
                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/week 2">week 2</a></li>
                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/week 3">week 3</a></li>
                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/week 3">week 3</a></li>
 +
                 </ul>
                 </ul>
      
      

Revision as of 08:52, 22 September 2012

SUSTC iGEM Theme - Free CSS Template

SUSTC iGEM Theme - Free CSS Template

September

Abstract

From this week we paid more attention to our human practices


Week 2

9.16.2012-9.22.2012

9.17.2012

After all the material we bought arrived, we started our lab work again in our school. It’s a hard work to develop our own laboratory. But we had fun at the same time.

We did a digest and amplify GFP & RFP with PCR.


9.18.2012-9.20.2012

We visited 3 high schools and gave presentations to their students on synthetic biology and our IGEM projects. There are many students being interested in biology and some of them would like to join this competition. We think our major goal has been achieved. We also did a ligation to connect GFP, RFP and the plasmid together. Then we did a transformation.


9.21.2012

Today’s morning, we saw that control group almost exist only few of bacterial colony and the experimental group grow lots of colonies. I think it is a positive suggest. So we did a colony PCR to test whether the ligation is successful. We picked up 10 colonies to test and three of them is positive ligated by three parts and all the colonies are positive ligated by two parts.


9.22.2012

We picked up those colonies which are positive. Through the bacterium culture in shake-flask more than 12 hours, we can do plasmid miniprep.


Week Diary