Team:SUSTC-Shenzhen-B/protocol

From 2012.igem.org

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                 <h2>Achievements</h2>
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                 <h2>Lab Protocol</h2>
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                 <div class="cleaner h40"></div>
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                 <h3>Software</h3>
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                 <h3>brief process</h3>
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<p>1. If users input the full sequence of terminator, our program can divide it into three parts, A tail, stemloop, and T tail.</p>
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<p>1. Site-Directed Mutagenesis </p>
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<p>2. By using Free energy(or Hairpin Score) Minimization Rule, we can get secondary structure of stemloop. Then we can output a picture of the terminator.</p>
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<p> 2. Restriction enzyme digestion and electrophoresis (proof)</p>
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<p>3. Because of the time limit, we will measure more than one hundred terminators to get the best fitting equation between score and terminator efficiency. So if users input a terminator sequence, we will output its predicted efficiency.</p>
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<p> 3. Media Preparation</p>
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<br>
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<p> 4. Bacterial Transformation</p>
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<p> 5. Colony PCR for verification</p>
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<p> 6.  Culture the bacteria  </p>
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<p> 7.  Plasmid DNA Isolation</p>
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<p> 8.  Restriction Enzyme Digestion and Electrophoresis(proof) </p>
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<p> 9. Amplify GFP & RFP</p>
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<p> 10. Electrophoresis and Restriction Enzyme Digestion.</p>
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<p>11. Ligation</p>
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<p>12. Bacterial Transformation</p>
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<p>13. bacterial colony PCR for verification</p>
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<p> 14. Culture the bacteria</p>
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<p> 15. Plasmid DNA Isolation</p>
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<p> 16. Restriction Enzyme Digestion and Electrophoresis</p>
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                 <h3>Database</h3>
                 <h3>Database</h3>
<p>颓哥加油写啊！</p>
<p>颓哥加油写啊！</p>

Revision as of 17:05, 23 September 2012

SUSTC iGEM Theme - Free CSS Template

SUSTC iGEM Theme - Free CSS Template

Lab Protocol

brief process

1. Site-Directed Mutagenesis

2. Restriction enzyme digestion and electrophoresis (proof)

3. Media Preparation

4. Bacterial Transformation

5. Colony PCR for verification

6. Culture the bacteria

7. Plasmid DNA Isolation

8. Restriction Enzyme Digestion and Electrophoresis(proof)

9. Amplify GFP & RFP

10. Electrophoresis and Restriction Enzyme Digestion.

11. Ligation

12. Bacterial Transformation

13. bacterial colony PCR for verification

14. Culture the bacteria

15. Plasmid DNA Isolation

16. Restriction Enzyme Digestion and Electrophoresis

Database

颓哥加油写啊！

Hard Work