Team:SUSTC-Shenzhen-B/lab results

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<h3 class="STYLE2"><font face="Arial, Helvetica">3.Agreement with theoretical prediction</font></h3>
<h3 class="STYLE2"><font face="Arial, Helvetica">3.Agreement with theoretical prediction</font></h3>
<p class="STYLE3"><span class="STYLE2"><img src="http://2012.igem.org/wiki/images/d/d5/Labresult.jpg" class="img_fl img_border" //font /></span></p>
<p class="STYLE3"><span class="STYLE2"><img src="http://2012.igem.org/wiki/images/d/d5/Labresult.jpg" class="img_fl img_border" //font /></span></p>
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<p class="STYLE3">(Figure 3 According to our  experiment measured terminator efficiencies and our software predicted dscores,  we create fit curve to relate all the data . To correspond to the figure 3, we  choose linear fit curve to link all the data. There are 5 valuable points of terminator  efficiencies. The sequences are listed below. In the experiment, we transform 9  terminators to plasmid mutant-psb1a3-GF  and only get 5 reliably terminators’ data. The points appeared on the figure  are terminators whose number is 1,4,6,7,8 )</p>
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<p class="STYLE3">(Figure 3 The horizontal ordinate represents the value of efficiencies measured by experiment. The vertical ordinate represents the value of efficiencies predicted by TTEC. We can see a good agreement between experimental efficiency and TTEC predicted efficiency.)</p>
<p class="STYLE4"><span class="STYLE2"><img src=" http://2012.igem.org/wiki/images/2/2b/94.png" class="img_fl img_border" //font /></span></p>
<p class="STYLE4"><span class="STYLE2"><img src=" http://2012.igem.org/wiki/images/2/2b/94.png" class="img_fl img_border" //font /></span></p>
<p class="STYLE3">(Figure 4 Here listed the sequences of  terminator whose efficiencies are measured in the lab.)</p>
<p class="STYLE3">(Figure 4 Here listed the sequences of  terminator whose efficiencies are measured in the lab.)</p>

Revision as of 20:07, 26 October 2012

Title

Result


1. Fluorescence microscope results

Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that GFP is expressed.

(Figure 1 Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. )

2.Flow cytometry results

Figure2-a

Figure2-b

Flow cytometry results of GFP expression in experiment group Figure2-a and control group Figure2-b.The average GFP fluorescence strength of control group is 432 unit and of experiment group is 251 unit. Therefore, the terminator efficiency is 251/432=0.58

3.Agreement with theoretical prediction

(Figure 3 The horizontal ordinate represents the value of efficiencies measured by experiment. The vertical ordinate represents the value of efficiencies predicted by TTEC. We can see a good agreement between experimental efficiency and TTEC predicted efficiency.)

(Figure 4 Here listed the sequences of terminator whose efficiencies are measured in the lab.)