Team:SUSTC-Shenzhen-B/lab results

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                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.  Fluorescence microscope results</font></h3>
                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.  Fluorescence microscope results</font></h3>
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Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures (Figure 1 and Figure 2) show that both GFP and RFP are expressed.  
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<p>Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that both GFP is expressed.</p>
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                <p class="STYLE2"><font face="Arial, Helvetica"><img src=" http://2012.igem.org/wiki/images/d/d2/71.png" class="img_fl img_border" //font></p>
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<p>488nm light was used to activate RFP. However we couldn’t see the expression of RFP.</p>
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<img src=" http://2012.igem.org/wiki/images/e/ec/81.png" class="img_fl img_border" >
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              <p><span class="STYLE3">(Figure 1: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the RFP. )</span></p>
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               <p><span class="STYLE3">(Figure 1: Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
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<p class="STYLE2"><font face="Arial, Helvetica"><img src=" http://2012.igem.org/wiki/images/e/ec/81.png" class="img_fl img_border" //font></p>
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               <p><span class="STYLE3">(Figure 2: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
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               <p>&nbsp;</p>
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               <h3 class="STYLE2"><font face="Arial, Helvetica">2. Fit curves relating to dscores and terminator  efficiencies. </font></h3>
               <h3 class="STYLE2"><font face="Arial, Helvetica">2. Fit curves relating to dscores and terminator  efficiencies. </font></h3>

Revision as of 16:45, 26 October 2012

Title

Result


1. Fluorescence microscope results

Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that both GFP is expressed.

488nm light was used to activate RFP. However we couldn’t see the expression of RFP.

(Figure 1: Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. )

 

2. Fit curves relating to dscores and terminator efficiencies.

(figure 6: Summary of results)

(figure 3 : According the data on biofab ( http://io.biofab.org/services/studio/dac/ ) and our software predicted dscores, create fit curves. Linear fit curve is founded to have the highest accuracy among all the fit curves. )

(figure 4 : According to our experiment measured terminator efficiencies and our software predicted dscores, we create fit curve to relate all the data . To correspond to the figure 3, we choose linear fit curve to link all the data. There are 5 valuable points of terminator efficiencies. The sequences are listed below. In the experiment, we transform 9 terminators to plasmid mutant-psb1a3-GF and only get 5 reliably terminators’ data. The points appeared on the figure are terminators whose number is 1,4,6,7,8 )

(figure 5 : Here listed the sequences of terminator whose efficiencies are measured in the lab.)