Team:SUSTC-Shenzhen-B/lab results

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              <h2><font face="Arial, Helvetica">
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                <h2>Lab Results:</h2>
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              <p>Result</p>
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                 <h3>What we have done:</h3>
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<p>1. We have successfully mutate the plasmid.</p>
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                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.   Pictures seen under fluorescence microscope</font></h3>
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<p>2. We have successfully digest the plasmid GFP and RFP.</p>
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<p>3. The ligation of these 3 fragments is successful.</p>
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              <p><span class="STYLE3">(figure 1: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the RFP. )</span></p>
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              <h3>Future Plan:</h3>
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              <p><span class="STYLE3">(figure 2: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
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<p>Because of the time limit, we still haven’t finished our experiment.</p>
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              <p>&nbsp;</p>
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<p>The next step of our experiment is to ligate the terminator to the backbone and then do a transformation, then test the fluorescent.</p>
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              <h3 class="STYLE2"><font face="Arial, Helvetica">2. Fit curves relating to dscores and terminator  efficiencies. </font></h3>
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<p class="STYLE3">(figure 3 : According the data on biofab ( <a href="http://io.biofab.org/services/studio/dac/">http://io.biofab.org/services/studio/dac/</a> ) and  our software predicted dscores,  create fit curves. Linear fit curve is founded to have the highest accuracy  among all the fit curves. )</p>
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<p class="STYLE3">(figure 4 : According to our  experiment measured terminator efficiencies and our software predicted dscores,  we create fit curve to relate all the data . To correspond to the figure 3, we  choose linear fit curve to link all the data. There are 5 valuable points of terminator  efficiencies. The sequences are listed below. In the experiment, we transform 9  terminators to plasmid mutant-psb1a3-GF  and only get 5 reliably terminators’ data. The points appeared on the figure  are terminators whose number is 1,4,6,7,8 )</p>
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<p class="STYLE3">(figure 5 : Here listed the sequences of  terminator whose efficiencies are measured in the lab.)</p>
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                <h2>Hard Work</h2>
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Revision as of 21:08, 26 September 2012

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Result


1.  Pictures seen under fluorescence microscope






















(figure 1: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the RFP. )
























(figure 2: Those bacteria who express GFP and RFP are seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. )

 

2. Fit curves relating to dscores and terminator efficiencies.

(figure 3 : According the data on biofab ( http://io.biofab.org/services/studio/dac/ ) and  our software predicted dscores, create fit curves. Linear fit curve is founded to have the highest accuracy among all the fit curves. )

(figure 4 : According to our experiment measured terminator efficiencies and our software predicted dscores, we create fit curve to relate all the data . To correspond to the figure 3, we choose linear fit curve to link all the data. There are 5 valuable points of terminator efficiencies. The sequences are listed below. In the experiment, we transform 9 terminators to plasmid mutant-psb1a3-GF and only get 5 reliably terminators’ data. The points appeared on the figure are terminators whose number is 1,4,6,7,8 )

(figure 5 : Here listed the sequences of terminator whose efficiencies are measured in the lab.)