Team:SUSTC-Shenzhen-B/lab results

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                                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li>
                                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li>
                                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li>
                                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li>
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                                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Achievements</a></li>
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                                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li>
                                     <li><a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li>
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                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.  Fluorescence microscope results</font></h3>
                 </font><h3 class="STYLE2"><font face="Arial, Helvetica">1.  Fluorescence microscope results</font></h3>
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Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures (Figure 1 and Figure 2) show that both GFP and RFP are expressed.  
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<p>Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that GFP is expressed.<p>
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                <p class="STYLE2"><font face="Arial, Helvetica"><img src=" http://2012.igem.org/wiki/images/d/d2/71.png" class="img_fl img_border" //font></p>
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<img src=" http://2012.igem.org/wiki/images/e/ec/81.png" class="img_fl img_border" >
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              <p><span class="STYLE3">(Figure 1 Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
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              <p><span class="STYLE3">(Figure 1: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the RFP. )</span></p>
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<h3 class="STYLE2"><font face="Arial, Helvetica">2.Flow cytometry results </font></h3>
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<p class="STYLE2"><font face="Arial, Helvetica"><img src=" http://2012.igem.org/wiki/images/e/ec/81.png" class="img_fl img_border" //font></p>
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<img src=" http://2012.igem.org/wiki/images/6/60/Lab_results-f1.jpg" class="img_fl img_border" >
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<p><span class="STYLE3">Figure2-a</span></p>
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              <p><span class="STYLE3">(Figure 2: Those bacteria who express GFP and  RFP are seen under the fluorescence microscope activated by 488nm light.The  picture shows the expression of the GFP. ) </span></p>
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<img src=" http://2012.igem.org/wiki/images/6/60/Lab_results-f2.jpg" class="img_fl img_border" >
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              <p>&nbsp;</p>
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<p><span class="STYLE3">Figure2-b</span></p>
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              <h3 class="STYLE2"><font face="Arial, Helvetica">2. Fit curves relating to dscores and terminator  efficiencies. </font></h3>
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<p>Flow cytometry results of GFP expression in experiment group Figure2-a and control group Figure2-b.The average GFP fluorescence strength of control group is 432 unit and of experiment group is 251 unit. Therefore, the terminator efficiency is 251/432=0.58</p>
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<p class="STYLE4"><span class="STYLE2"><img src=" http://2012.igem.org/wiki/images/6/6d/QQ%E6%88%AA%E5%9B%BE20120927115928.png" class="img_fl img_border" //font /></span></p>
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<h3 class="STYLE2"><font face="Arial, Helvetica">3.Agreement with theoretical prediction</font></h3>
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<p class="STYLE3">(figure 6:  Summary of results)</p>
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<p class="STYLE3"><span class="STYLE2"><img src="http://2012.igem.org/wiki/images/d/d5/Labresult.jpg" class="img_fl img_border" //font /></span></p>
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<p class="STYLE2"><img src="http://2012.igem.org/wiki/images/e/ec/91.png" class="img_fl img_border" //font /></p>
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<p class="STYLE3">(Figure 3 The horizontal ordinate represents the value of efficiencies measured by experiment. The vertical ordinate represents the value of efficiencies predicted by TTEC. We can see a good agreement between experimental efficiency and TTEC predicted efficiency.)</p>
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<p class="STYLE3">(figure 3 : According the data on biofab ( <a href="http://io.biofab.org/services/studio/dac/">http://io.biofab.org/services/studio/dac/</a> ) and  our software predicted dscores,  create fit curves. Linear fit curve is founded to have the highest accuracy  among all the fit curves. )</p>
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<p class="STYLE3"><span class="STYLE2"><img src=" http://2012.igem.org/wiki/images/d/d6/92.png" class="img_fl img_border" //font /></span></p>
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<p class="STYLE3">(figure 4 : According to our  experiment measured terminator efficiencies and our software predicted dscores,  we create fit curve to relate all the data . To correspond to the figure 3, we  choose linear fit curve to link all the data. There are 5 valuable points of terminator  efficiencies. The sequences are listed below. In the experiment, we transform 9  terminators to plasmid mutant-psb1a3-GF  and only get 5 reliably terminators’ data. The points appeared on the figure  are terminators whose number is 1,4,6,7,8 )</p>
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<p class="STYLE4"><span class="STYLE2"><img src=" http://2012.igem.org/wiki/images/2/2b/94.png" class="img_fl img_border" //font /></span></p>
<p class="STYLE4"><span class="STYLE2"><img src=" http://2012.igem.org/wiki/images/2/2b/94.png" class="img_fl img_border" //font /></span></p>
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<p class="STYLE3">(figure 5 : Here listed the sequences of  terminator whose efficiencies are measured in the lab.)</p>
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<p class="STYLE3">(Figure 4 Here listed the sequences of  terminator whose efficiencies are measured in the lab.)</p>
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Latest revision as of 21:18, 26 October 2012

Title

Result


1. Fluorescence microscope results

Our plasmid design contains a RFP and GFP. Fluorescence microscope pictures Figure 1 shows that GFP is expressed.

(Figure 1 Those bacteria who express GFP is seen under the fluorescence microscope activated by 488nm light.The picture shows the expression of the GFP. )

2.Flow cytometry results

Figure2-a

Figure2-b

Flow cytometry results of GFP expression in experiment group Figure2-a and control group Figure2-b.The average GFP fluorescence strength of control group is 432 unit and of experiment group is 251 unit. Therefore, the terminator efficiency is 251/432=0.58

3.Agreement with theoretical prediction

(Figure 3 The horizontal ordinate represents the value of efficiencies measured by experiment. The vertical ordinate represents the value of efficiencies predicted by TTEC. We can see a good agreement between experimental efficiency and TTEC predicted efficiency.)

(Figure 4 Here listed the sequences of terminator whose efficiencies are measured in the lab.)